pLenti-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector

CAT＃: PS100092

pLenti-C-Myc-DDK-P2A-Puro, Lenti vector with C-terminal Myc-DDK tag and P2A-Puro, 10 ug

Datasheet
SDS
Vector Sequence

CNY 7,130.00

货期*
现货
规格
    • 10 ug
Cited in 37 publications.

Product images

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Specifications

Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Cell Selection Puromycin
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with C-terminal Myc-DDK tags.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (37)

The use of this Vectors has been cited in the following citations:
CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma ,null, Cell Death and Differentiation ,PubMed ID 37468549
BRD9 Degradation Disrupts Ribosome Biogenesis in Multiple Myeloma ,null, Clinical Cancer Research ,PubMed ID 36780189
Hyperandrogenism diminishes maternal–fetal fatty acid transport by increasing FABP4-mediated placental lipid accumulation ,null, Biology of Reproduction ,PubMed ID 35357467
Neuronal Prosurvival Role of Ceramide Synthase 2 by Olidogendrocyte-to-Neuron Extracellular Vesicle Transfer ,null, International Journal of Molecular Sciences ,PubMed ID 36983060
Loss of IGFBP2 mediates alveolar type 2 cell senescence and promotes lung fibrosis ,null, Cell Reports Medicine ,PubMed ID 36787736
Aberrant hyperexpression of the RNA binding protein FMRP in tumors mediates immune evasion. ,null, Science (New York, N.Y.) ,PubMed ID 36395212
Reformation of the chondroitin sulfate glycocalyx enables progression of AR-independent prostate cancer ,null, Nature Communications ,PubMed ID 35963852
Single-cell RNA sequencing identifies G-protein coupled receptor 87 as a basal cell marker expressed in distal honeycomb cysts in idiopathic pulmonary fibrosis ,null, The European Respiratory Journal ,PubMed ID 35604813
Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδ ,null, Nature Communications ,PubMed ID 35562376
Antisense Oligonucleotide-Mediated Silencing of Mitochondrial Fusion and Fission Factors Modulates Mitochondrial Dynamics and Rescues Mitochondrial Dysfunction ,null, Nucleic Acid Therapeutics ,PubMed ID 34698563
Upregulation of CD36, a Fatty Acid Translocase, Promotes Colorectal Cancer Metastasis by Increasing MMP28 and Decreasing E-Cadherin Expression ,null, Cancers ,PubMed ID 35008415
Peroxidasin Enhances Basal Phenotype and Inhibits Branching Morphogenesis in Breast Epithelial Progenitor Cell Line D492 ,null, Journal of Mammary Gland Biology and Neoplasia ,PubMed ID 34964086
Association of Variants in the SPTLC1 Gene With Juvenile Amyotrophic Lateral Sclerosis ,null, JAMA Neurology ,PubMed ID 34459874
The microRNA-210-Stathmin1 Axis Decreases Cell Stiffness to Facilitate the Invasiveness of Colorectal Cancer Stem Cells ,Liao, T;Cheng, W;Yang, C;Chen, Y;Su, S;Yeh, T;Lan, H;Lee, C;Lin, H;Lin, C;Lu, R;Chiou, A;Jiang, J;Hwang, W;, Cancers ,PubMed ID 33921319 [pLenti-C-Myc-DDK-P2A-Puro]
Recombinant apoptosis inhibitor of macrophage protein reduces delayed graft function in a murine model of kidney transplantation ,Lee, JY;Arumugarajah, S;Lian, D;Maehara, N;Haig, AR;Suri, RS;Miyazaki, T;Gunaratnam, L;, PloS one ,PubMed ID 33891625 [pLenti-C-Myc-DDK-P2A-Puro]
A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes ,Wang, TY;Liu, Q;Ren, Y;Alam, SK;Wang, L;Zhu, Z;Hoeppner, LH;Dehm, SM;Cao, Q;Yang, R;, Molecular cell ,PubMed ID 33861991 [pLenti-C-Myc-DDK-P2A-Puro]
Activation of the eIF2α/ATF4 axis drives triple-negative breast cancer radioresistance by promoting glutathione biosynthesis ,null, Redox Biology ,PubMed ID 33946018
Further evidence supporting a potential role for ADH1B in obesity ,Morales, LD;Cromack, DT;Tripathy, D;Fourcaudot, M;Kumar, S;Curran, JE;Carless, M;Göring, HHH;Hu, SL;Lopez-Alvarenga, JC;Garske, KM;Pajukanta, P;Small, KS;Glastonbury, CA;Das, SK;Langefeld, C;Hanson, RL;Hsueh, WC;Norton, L;Arya, R;Mummidi, S;Blangero, J;DeFronzo, RA;Duggirala, R;Jenkinson, CP;, Scientific reports ,PubMed ID 33479282 [pLenti-C-Myc-DDK-P2A-Puro]
Reciprocal activation of HEY1 and NOTCH4 under SOX2 control promotes EMT in head and neck squamous cell carcinoma ,Fukusumi, T;Guo, T;Ren, S;Haft, S;Liu, C;Sakai, A;Ando, M;Saito, Y;Sadat, S;Califano, J;, International Journal of Oncology ,PubMed ID 33491747 [pLenti-C-Myc-DDK-P2A-Puro]
Human CLPB forms ATP-dependent complexes in the mitochondrial intermembrane space. ,null, The international journal of biochemistry cell biology ,PubMed ID 32866687
Dianhydrogalactitol synergizes with topoisomerase poisons to overcome DNA repair activity in tumor cells ,Zhai, B;Li, Y;Kotapalli, SS;Bacha, J;Brown, D;Steinø, A;Daugaard, M;, Cell Death Dis ,PubMed ID 32709853 [pLenti-C-Myc-DDK-P2A-Puro]
Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells ,Zhang, X;Duan, L;Zhang, Y;Zhao, H;Yang, X;Zhang, C;, Oncol Lett ,PubMed ID 32724439 [pLenti-C-Myc-DDK-P2A-Puro]
Differential glucocorticoid-dependent regulation and function of the ERRFI1 gene in triple-negative breast cancer ,Mojica, CAR;Ybañez, WS;Olarte, KCV;Poblete, ABC;Bagamasbad, PD;, Endocrinology ,PubMed ID 32432675 [pLenti-C-Myc-DDK]
A kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury ,Kim, JY;Bai, Y;Jayne, LA;Hector, RD;Persaud, AK;Ong, SS;Rojesh, S;Raj, R;Feng, MJHH;Chung, S;Cianciolo, RE;Christman, JW;Campbell, MJ;Gardner, DS;Baker, SD;Sparreboom, A;Govindarajan, R;Singh, H;Chen, T;Poi, M;Susztak, K;Cobb, SR;Pabla, NS;, Nat Commun ,PubMed ID 32317630 [pLenti-C-Myc-DDK-P2A-Puro]
Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway ,Wang, Q;Zhang, N;Hu, L;Xi, Y;Mi, W;Ma, Y;, Tissue Eng Regen Med ,PubMed ID 32200515 [pLenti-C-Myc-DDK- P2A-Puro]
mTORC2/Rac1 Pathway Predisposes Cancer Aggressiveness in IDH1-Mutated Glioma ,Liu, Y;Lu, Y;Li, A;Celiku, O;Han, S;Qian, M;Yang, C;, Cancers (Basel) 2020 ,PubMed ID 32224866 [pLenti-C-Myc-DDK-P2A-Puro]
Wnt signaling modulator DKK1 as an immunotherapeutic target in ovarian cancer. ,null, Gynecologic oncology ,PubMed ID 32192732
Oncogenic ERG represses PI3K signaling through down-regulation of IRS2 ,Mao, N;Gao, D;Hu, W;Gadal, S;Hieronymus, H;Wang, S;Lee, YS;Sullivan, P;Zhang, Z;Choi, D;Rosen, N;Sawyers, CL;Gopalan, A;Chen, Y;Carver, BS;, Cancer Res. ,PubMed ID 32015092 [pLenti-C-Myc-DDK-P2A-Puro]
PEDF-Rpsa-Itga6 signaling regulates cortical neuronal morphogenesis ,Blazejewski, SM;Bennison, SA;Ha, NT;Liu, X;Smith, TH;, bioRxiv [pLenti-C-Myc-DDK-P2A-Puro]
Peroxiredoxin 5 Silencing Sensitizes Dopaminergic Neuronal Cells to Rotenone via DNA Damage-Triggered ATM/p53/PUMA Signaling-Mediated Apoptosis ,Wang, MJ;Huang, HY;Chiu, TL;Chang, HF;Wu, HR;, Cells ,PubMed ID 31861721 [pLenti-C-Myc-DDK-P2A-Puro]
Amyloid-beta impairs TOM1-mediated IL-1R1 signaling ,null, Proceedings of the National Academy of Sciences of the United States of America ,PubMed ID 31570577
Mutations in the SPTLC1 gene are a cause of amyotrophic lateral sclerosis that may be amenable to serine supplementation ,Johnson, JO;Chia, R;Kumaran, R;Alahmady, N;, BioRxiv [pLenti-C-Myc-DDK-P2A-Puro]
Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer ,Su, H;Ren, F;Jiang, H;Chen, Y;, Oncology Letters ,PubMed ID 31452811 [pLenti-C-Myc-DDK-P2A-Puro]
Mutations in the Sphingolipid Pathway Gene SPTLC1 are a Cause of Amyotrophic Lateral Sclerosis ,Janel, J;Chia, R;Kumaran, R;Alahmady, N;Abramzon, Y;Faghri, F;Renton, A;Topp, S;Pliner, H;Gibbs, J;Ding, J;Smith, N;Landeck, N;Nalls, M;Cookson, M;Pletnikova, O;Troncoso, J;Scholz, S;Sabir, M;Ahmed, S;Dalgard, C;Troakes, C;Jones, A;Shatunov, A;Iacoangeli, A;Al Khleifat, A;Ticozzi, N;Silani, V;Gellera, C;Blair, I;Dobson-Stone, C;Kwok, J;England, B;Consortium, T;Consortium, T;Consortium, T;Center, T;Tienari, P;Stone, D;Morrison, K;Shaw, P;Al-Chalabi, A;Brown Jr., R;Brunetti, M;Calvo, A;Mora, G;Gotkine, M;Shaw, C;Landers, J;Chiò, A;Crawford, T;Smith, B;Traynor, B;, SSRN Journal [pLenti-C-Myc-DDK-P2A-Puro]
ONECUT2 is a driver of neuroendocrine prostate cancer ,Guo, H;Ci, X;Ahmed, M;Hua, JT;Soares, F;Lin, D;Puca, L;Vosoughi, A;Xue, H;Li, E;Su, P;Chen, S;Nguyen, T;Liang, Y;Zhang, Y;Xu, X;Xu, J;Sheahan, AV;Ba-Alawi, W;Zhang, S;Mahamud, O;Vellanki, RN;Gleave, M;Bristow, RG;Haibe-Kains, B;Poirier, JT;Rudin, CM;Tsao, MS;Wouters, BG;Fazli, L;Feng, FY;Ellis, L;van der Kwast, T;Berlin, A;Koritzinsky, M;Boutros, PC;Zoubeidi, A;Beltran, H;Wang, Y;He, HH;, Nat Commun ,PubMed ID 30655535 [pLenti-C-Myc-DDK-P2A-Puro]
Pleotropic effects of PPARD accelerate colorectal tumor progression and invasion ,Liu, Y;Deguchi, Y;Tian, R;Wei, D;Chen, W;Xu, M;Wu, L;Liu, F;Gao, S;Jaoude, J;Chrieki, S;Moussalli, M;Gagea, M;Morris, J;Broaddus, R;Zuo, X;Shureiqi, I;, bioRxiv [pLenti-C-Myc-DDK-P2A-Puro]
mTORC2/AKT/HSF1/HuR constitute a feed-forward loop regulating Rictor expression and tumor growth in glioblastoma ,null, Oncogene ,PubMed ID 29059166

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