C18orf1 (LDLRAD4) Human shRNA Plasmid Kit (Locus ID 753)

Specifications

Product Data
Product Name	C18orf1 (LDLRAD4) Human shRNA Plasmid Kit (Locus ID 753)
Locus ID	753
UniProt ID	O15165
Synonyms	C18orf1
Vector	pRS
Format	Retroviral plasmids
Kit Components	LDLRAD4 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 753). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001003674, NM_001003675, NM_001276249, NM_001276251, NM_004338, NM_181481, NM_181482, NM_181483, NM_001003675.1, NM_001003675.2, NM_001003675.3, NM_181483.1, NM_181483.2, NM_181483.3, NM_181482.1, NM_181482.2, NM_181482.3, NM_181482.4, NM_004338.1, NM_004338.3, NM_001003674.1, NM_001003674.2, NM_001003674.3, NM_181481.1, NM_181481.2, NM_181481.3, NM_181481.4, NM_001276251.1, NM_001276249.1, BC029958, BC030199, BC047093, BC066971, BM715555, BM805426, BM917439, BM991627, NM_181481.5, NM_181482.5
Summary	Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.