Escherichia coli Recombinant Protein
CAT#: TP724706
Recombinant E.coli RNA-splicing ligase RtcB
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CNY 3,140.00
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Specifications
Product Data | |
Species | Escherichia coli |
Protein Source | E. coli |
Expression cDNA Clone or AA Sequence |
M1-G408
|
Buffer | Supplied as a 0.2 um filtered solution of 20mM HEPES, 300mM NaCl,10%glycerol, 2mM DTT,0.1mM EDTA,pH7.5. |
Note | Recombinant E.coli RNA-splicing ligase RtcB is produced by E.coli. The target gene encoding M1-G408 is expressed. |
Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
Stability | 12 months from date of despatch |
Reference Data | |
Synonyms | 3'-phosphate/5'-hydroxy nucleic acid ligase; rtcB; yhgL |
Summary | RNA-splicing ligase RtcB is a member of RtcB family. It is a GTP-dependent RNA ligase that is involved in tRNA splicing and RNA repair. RtcB rejoins broken stem-loops of tRNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends. RtcB can also act as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-phosphate (or cyclic-phosphate) and 5'-hydroxy ends that cannot be sealed by classical DNA ligases. |
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