ARIH1 (NM_005744) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) 1         MDSDEGYNYE FDEDEECSEE  21        DSGAEEEEDE DDDEPDDDTL  41        DLGEVELVEP GLGVGGERDG  61        LLCGETGGGG GSALGPGGGG  81        GGGGGGGGPG HEQEEDYRYE  101       VLTAEQILQH MVECIREVNE  121       VIQNPATITR ILLSHFNWDK  141       EKLMERYFDG NLEKLFAECH  161       VINPSKKSRT RQMNTRSSAQ  181       DMPCQICYLN YPNSYFTGLE  201       CGHKFCMQCW SEYLTTKIME  221       EGMGQTISCP AHGCDILVDD  241       NTVMRLITDS KVKLKYQHLI  261       TNSFVECNRL LKWCPAPDCH  281       HVVKVQYPDA KPVRCKCGRQ  301       FCFNCGENWH DPVKCKWLKK  321       WIKKCDDDSE TSNWIAANTK  341       ECPKCHVTIE KDGGCNHMVC  361       RNQNCKAEFC WVCLGPWEPH  381       GSAWYNCNRY NEDDAKAARD  401       AQERSRAALQ RYLFYCNRYM  421       NHMQSLRFEH KLYAQVKQKM  441       EEMQQHNMSW IEVQFLKKAV  461       DVLCQCRATL MYTYVFAFYL  481       KKNNQSIIFE NNQADLENAT  501       EVLSGYLERD ISQDSLQDIK  521       QKVQDKYRYC ESRRRVLLQH  541       VHEGYEKDLW EYIED
Tag	C-Myc/DDK
Predicted MW	63.9 kDa
Concentration	>0.05 µg/µL as determined by microplate Bradford method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Storage	Store at -80°C after receiving vials.
Stability	Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_005735
Locus ID	25820
UniProt ID	Q9Y4X5
Refseq Size	2178
Cytogenetics	15q24.1
Refseq ORF	1671
Synonyms	ARI; HARI; HHARI; UBCH7BP
Summary	E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916).[UniProtKB/Swiss-Prot Function]
Protein Families	Druggable Genome

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