Trim50 (NM_178240) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR224711 representing NM_178240 Red=Cloning site Green=Tags(s) MAWRLTVPELQDQLQCPICLEVFKEPLMLQCGHSYCKDCLDNLSQHLDSELCCPVCRQSVDCSSSPPNVS LARVIDALRLPGDIEPTVCVHHRNPLSLFCEKDQEFICGLCGLLGSHQHHRVTPVSTVYSRMKEELAGRI SELKEEHRNVEEHIGKLVNNRTRIINESDVFSWVIRREFQELHHLVDEEKARCLEGLEGHTRGLVASLDM QLEQAQGTQERLAQAEQVLEQFGNESHHEFIRFHSVASRAEVQQARPLEGVFSPISFKPALHQADIKLTV WKRLFRKVLPAPASLKLDPATAHPLLELSKGNTVVHCGLLAQRRASQPERFDYSTCVLASKGFSWGRHYW EVVVGSKSDWRLGVIKGTASRKGKLNKSPEHGVWLIGLKEGRVYEAFGCPRLPLPVAGHPHRIGVYLHYE QGELTFFDADRPDDLRTLYTFQADFQGKLYPILDTCWHERGSNSLPMVLPPPSGPGHFTLGQV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	55.1 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_839971
Locus ID	215061
UniProt ID	Q810I2
Refseq Size	1452
Cytogenetics	5 G2
Refseq ORF	1449
Summary	E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.