Ube3c (NM_133907) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR223355 protein sequence Red=Cloning site Green=Tags(s) MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAVIIQSFIRGYRDRKQQYFIQR SAFDQCTDSAQPGGTFCLADGPNLTLLVRQLLFFYKQSEDSKRLIWLYQNLIKHSSLFVKQLDGSERLTC LFQIKRLMSLCCRLLQNCSDDSLNVALPMRMLEVFTSENTYLPVLQDSSYVVSVIEQILHYMVHSGYYRS LYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYSSCPEASRHQVFSAFTEEFLGAPFTDQIFHF VIPAFADAQTVFPYEPFLNALLLLESQSSKRCSGVPWLFYFVLTVGENYLGALSEDGLLVYLRVLQTFLS QLPASPTGTGCPDSTSDSEDDNEETDQPNSPEDGRVSAPYITEECLRKLDTKQQTNTLLNLVWRDSASEE VFTRMASICHTLMVQHRMMVPKVRLLYSLAFNARFLRHLWFLISSMTTQMITGSMVPLLQLISRGSPMSF EDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELILLSRCLRDACLGIIKLA YPETKPEVREEYVTAFQSIGVTTNSEMQQCIQMEQKRWVQLFKVITNLVKMLKSRDTRRNFCPPNHWLSE QEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSTLEVGLESLPLSVSEERQLAILTELPFVVPFEERVK IFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGG GIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFA GFFLSKLLGTSADVDIHHLASLDPEVYRNLLFLKSYEEDVEELGLNFTVVNNDLGEAQVVELKFGGKDIP VTGANRIAYIHLVADYRLNKQIRPHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPVSLEDLKSFT NYSGGYSADHPVIKIFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTAST CMNLLKLPEFYDEALLRSKLLYAIECAAGFELS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	124 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_598668
Locus ID	100763
UniProt ID	Q80U95
Refseq Size	5033
Cytogenetics	5 B1
Refseq ORF	3252
Synonyms	AI853514; mKIAA0010
Summary	E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.[UniProtKB/Swiss-Prot Function]

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