Dock8 (NM_028785) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR219092 representing NM_028785 Red=Cloning site Green=Tags(s) MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSISTSGFPSLQLPQLYEPVEPVDFEGLVMT HLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSS EFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDER LENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEI EIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKI EKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLER ESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLA DYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVY RNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYE EVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSI HSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTL EHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRK DQHGRNCLLASYVHYVFRLPELHRDTSKSGGPITVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLT GSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETV FKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKIN ISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTDTA PASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAI HSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNV ALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILD LLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWR KEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSL SCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFG ATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYD TVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCFTEAAMCLVHAAALVAEYLSML EDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVN EVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSRFGDLDEQEFVYKEPAI TKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFN LRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQL AVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVE KNRRLITAEQREYQQELKKNYNKLRDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	239.4 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_083061
Locus ID	76088
UniProt ID	Q8C147
Refseq Size	7810
Cytogenetics	19 B
Refseq ORF	6300
Synonyms	1200017A24Rik; 5830472H07Rik; A130095G14Rik; AI461977
Summary	Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:28028151, PubMed:22461490). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (PubMed:22461490, PubMed:25713392). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151). Is involved in NK cell cytotoxicity controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (By similarity).[UniProtKB/Swiss-Prot Function]

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