Kdm3a (NM_001038695) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR211928 representing NM_001038695 Red=Cloning site Green=Tags(s) MVLTLGESWPVLVGKRFLSLSAAEGNEGGQDNWDLERVAEWPWLSGTIRAVSHTDVTKKDLKVCVEFDGE SWRKRRWIDVYSLQRKAFLVEHNLVLAERKSPEVPEQVIQWPAIMYKSLLDKAGLGAITSVRFLGDQQSV FVSKDLLKPIQDVNSLRLSLTDNQTVSKEFQALIVKHLDESHLLQGDKNLVGSEVKIYSLDPSTQWFSAT VVHGNPSSKTLQVNCEEIPALKIVDPALIHVEVVHDNFVTCGNSTRTGAVKRKSSENNGSSVSKQAKSCS EASPSMCPVQSVPTTVFKEILLGCTAATPSSKDPRQQNTPQAANSPPNIGAKLPQGCHKQNLPEELSSCL NTKPEVPRTKPDVCKEGLLSSKSSQVGAGDLKILSEPKGSCIQPKTNTDQESRLESAPQPVTGLPKECLP AKTSSKAELDIATTPELQKHLEHAASTSDDLSDKPEVKAGVTSLNSCAEKKVEPSHLGSQSQNLKETSVK VDNESCCTRSSNKTQTPPARKSVLTDPDKVRKLQQSGEAFVQDDSCVNIVAQLPKCRECRLDSLRKDKDQ QKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDSEAIGLWLPLTKNVVGTDLDTAKYILANIGDHFC QMVISEKEAMSTIEPHRQVAWKRAVKGVREMCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAA YKTFSWIRCVKSQIHEPENLMPTQIIPGKALYDVGDIVHSVRAKWGIKANCPCSNRQFKLFSKPALKEDL KQTSLSGEKPTLGTMVQQSSPVLEPVAVCGEAASKPASSVKPTCPTSTSPLNWLADLTSGNVNKENKEKQ LTMPILKNEIKCLPPLPPLNKPSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTAKTENGLKNTPKILDDI FASLVQNKTSSDSSKRPQGLTIKPSILGFDTPHYWLCDNRLLCLQDPNNKSNWNVFRECWKQGQPVMVSG VHHKLNTELWKPESFRKEFGEQEVDLVNCRTNEIITGATVGDFWDGFEDVPNRLKNDKEKEPMVLKLKDW PPGEDFRDMMPSRFDDLMANIPLPEYTRRDGKLNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTN LHLDVSDAANVMVYVGIPKGQCEQEEEVLRTIQDGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREF LKKVSEEQGQDNPADHDPIHDQSWYLDRSLRKRLYQEYGVQGWAIVQFLGDVVFIPAGAPHQVHNLYSCI KVAEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIYHAVKDAVAMLKASESSLGKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	148.3 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_001033784
Locus ID	104263
UniProt ID	Q6PCM1
Refseq Size	4829
Cytogenetics	6 C1
Refseq ORF	3969
Synonyms	1700105C21Rik; C230043E16Rik; JHDM2a; Jmjd1; Jmjd1a; KDM2A; TGSA; Tsga
Summary	Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation (By similarity). Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin (PubMed:17943087). Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.[UniProtKB/Swiss-Prot Function]

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