Wnk4 (NM_175638) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR211822 representing NM_175638 Red=Cloning site Green=Tags(s) MLAPRNTETGVPMSQTEADLALRPSPALTSTGPTRLGPPPRRVRRFSGKAEPRPRSSRPSRRSSVDLGLL SSWSQPASLLPEPPDPPDSAGPTRSPPSSSKEPPEGTWMGAAPVKAVDSACPELTGSSGGPGSREPPRVP DAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRA ERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQ ILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYD EAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTI QDLLAHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMV ALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSLAPGPPSAFPPEPEEPEA DQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGLPSSPAESHLCLPSGFALSIPRSGPG SDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFR FDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPPEAAEDALSPQEEPAALPAL PGPPNAEPQRSISPEQRSWAAFSTSPSSPGTPLSPGAPFSPGTPPVFPCPIFPITSPSCYPCPFSQVSSN PYPQAPSSLLPLSSSASQVPLPSSSLPISAPLPFSPSYPQDPLSPTSLPVCPSPPSLPSTTAAPLLSLAS AFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPLSLASCDQESLSAQTAETENEASRNPAQPLLGDA RLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPSPPLTSESSDTEDSAAGGPETREALA ESDRAAEGLGVAVDDEKDEGKEPLLGGSSPILSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNL RQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPG IMRRNSLSGSSTGSQEQRASKGVTFAGDIGRM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	132.9 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_783569
Locus ID	69847
UniProt ID	Q80UE6
Refseq Size	4148
Cytogenetics	11 D
Refseq ORF	3666
Synonyms	2010002J11Rik; Pha2b; Prkwnk4
Summary	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537).[UniProtKB/Swiss-Prot Function]

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