Usp28 (NM_175482) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR211535 representing NM_175482 Red=Cloning site Green=Tags(s) MTAELQQDDSAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDT TAAEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQADNRDLNRAHEANSAETKRSKRK RCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYNLPQNILENCRSHTEKR NIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSH LRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVK YGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYKSKELIRSKRESVRKLKEEI QVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSAEHVTLPLPSVHCPISDLTPKESS SPESCSQNAGSTFSSPEDALPSSEGMNGPFTSPHSSLETPAPPAPRTVTDEEMNFVKTCLQRWRSEIEQD IQDLKNCISSSTKAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQTWLKYNDISVTESS WEELERDSYGGLRNVSAYCLMYINDNLPHFSAEASSNESDETAGEVEALSVELRQYIQEDNWRFQQEVEE WEEEQSCKIPQMESSPNSSSQDFSTSQESPAVSSHEVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEA ALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMK VAQAKLMEIGPDDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGP RRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVM RNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCNRFAAVMESIQGVSTVTV K TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	119.8 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_780691
Locus ID	235323
UniProt ID	Q5I043
Refseq Size	4220
Cytogenetics	9 A5.3
Refseq ORF	3153
Synonyms	9830148O20Rik; AU022237; mKIAA1515
Summary	Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.[UniProtKB/Swiss-Prot Function]

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