Dgcr8 (NM_033324) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR210572 protein sequence Red=Cloning site Green=Tags(s) METYESPSPLPREPAGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFY GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGV ERSTRPECGQLLSPVSGDVHACPFGGSVGNGVGLGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLE LDEEGTGGFTAKAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETS VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI PCLHYKKMKDNEEREQNCDLAPSGEVSPVKPLGRSAELDFPLEEPDSMGGDSGSMDEKDPLGAEAAAGAL GQVKAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLI TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASS KKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKR NHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGR ESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCT VDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	86.8 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_201581
Locus ID	94223
UniProt ID	Q9EQM6
Refseq Size	4226
Cytogenetics	16 11.31 cM
Refseq ORF	2322
Synonyms	D16H22S788E; D16H22S1742E; D16Wis2; Gy1; mir-1306; N41; Vo59c07
Summary	Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis (PubMed:17259983). Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (PubMed:17259983).[UniProtKB/Swiss-Prot Function]

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