Polk (BC052820) Mouse Recombinant Protein
CAT#: TP510382
Purified recombinant protein of Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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CNY 2,900.00
货期*
4周
规格
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经常一起买 (1)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
CNY 600.00
Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR210382 protein sequence
Red=Cloning site Green=Tags(s) MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYEYDPNFMAMSLDEAYLNITQH LQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNP QIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIK DLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMS VERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAI AKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGGSLDKTAKTELAK PLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSQSFETSENSNDCQTFICPVCF REQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITSVDGV DLTGTEDRSLKAASMDTLENNRSKEECPDIPDKSCPISLANETISTLSRQESVQPCTDEVVTGRALVCPV CNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPRGATSPSAPP CLATMVPLMLPYLPVCISVSCEQMQLELAMMSMKNISLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 82.4 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
Locus ID | 27015 |
UniProt ID | Q9QUG2 |
Refseq Size | 3517 |
Cytogenetics | 13 D1 |
Refseq ORF | 2217 |
Synonyms | Dinb1 |
Summary | DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:12432099). Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
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