Pomk (NM_029037) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR205284 protein sequence Red=Cloning site Green=Tags(s) MGQQHGTRNGLTHRELPRGVGLLLAMALMNVALYLCLDQLFISPGRSTADSRRCPPGYFRMGRMRNCSRW LSCEELRTEVRQLKRVGEGAVKRVFLSEWKEHKVALSRLTRLEMKEDFLHGLQMLKSLQSEHVVTLVGYC EEDGTILTEYHPLGSLSNLEETLNLSKYQDVNTWQHRLQLAMEYVSIINYLHHSPLGTRVMCDSNDLPKT LSQYLLTSNFSIVANDLDALPLVDHDSGILIKCGHRELHGDFVAPEQLWPYGEDTPFQDDLMPSYNEKVD IWKIPDVSSFLLGHVEGSDMVRFHLFDIHKACKSQIPAKRPTAQNVLDAYQRVFHSLRDTVMSQTKEML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	40 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_083313
Locus ID	74653
UniProt ID	Q3TUA9
Refseq Size	3601
Cytogenetics	8 A2
Refseq ORF	1050
Synonyms	4930444A02Rik; Sgk196
Summary	Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif (By similarity).[UniProtKB/Swiss-Prot Function]

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