Bcdin3d (NM_029236) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR203874 protein sequence Red=Cloning site Green=Tags(s) MAADGTLSRGGVGEAVEEEHPGALEPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPPEGPEKRPIL GLDVGCNSGDLSVALYKHFLSPRDGETCSGASRELRILCCDIDPVLVERAERDCPFPEALTFITLDIMDQ ESRKVPLSSFLSQFGRSVFDMVFCMSVTMWIHLNHGDRGLCEFLAHVSSLCSYLLVEPQPWKCYRAAARR LRKLGLHSFDHFRSLAIRGDMAKQIVRILTQDHGMELACCFGNTSWDRSLLLFRAKHTHETQAIPESSTK ETRTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	32 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_083512
Locus ID	75284
UniProt ID	Q91YP1
Refseq Size	1271
Cytogenetics	15 F1
Refseq ORF	858
Synonyms	4930556P03Rik; AV138748
Summary	O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA, acting as a capping enzyme. Less efficiently, also methylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs. There is some controversy about the methylation of pre-miR-145, since the dimethylation first described as the specific enzymatic activity cannot be reproduced by a more recent work which observes a monomehtylation of pre-miR-145 but two orders weaker than the methylation of cytosolic histidyl tRNA.[UniProtKB/Swiss-Prot Function]

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