dnaK / Hsp70 (Substrate binding domain) Escherichia coli Protein

Specifications

Product Data
Species	Escherichia coli
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST
Predicted MW	18 kDa
Concentration	lot specific
Purity	>95% by SDS-PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 25 mM Tris-HCl, pH 7.5, 2 mM β-mercaptoethanol, 1 mM EDTA
Preparation	Liquid purified protein
Protein Description	The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
Storage	Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
Summary	DNAK, originally identified for its DNA replication by bacteriophage λ in E. coli is the bacterial hsp70 chaperone.

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