L-asparaginase 1 (1-338, His-tag) Escherichia coli Protein
CAT#: AR39017PU-L
L-asparaginase 1 (1-338, His-tag) e. coli recombinant protein, 0.5 mg
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CNY 14,920.00
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Specifications
Product Data | |
Species | Escherichia coli |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGSSHHHHHH SSGLVPRGSH MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD
|
Tag | His-tag |
Predicted MW | 39.3 kDa |
Concentration | lot specific |
Purity | >95% |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2 mM DTT |
Preparation | Liquid purified protein |
Protein Description | Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
Summary | Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA is involved in intracellular asparagine utilization. AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. |
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