Podoplanin Mouse Protein

Specifications

Product Data
Species	Mouse
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) GTIGVNEDDI VTPGTGDGMV PPGIEDKITT TGATGGLNES TGKAPLVPTQ RERGTKPPLE ELSTSATSDH DHREHESTTT VKVVTSHSVD KKTSHPNRDN AGDETQTTDK KDGLPVVTLE HHHHHH N-terminal sequence: GTIGVNEDDI VTPGT
Predicted MW	13.42 kDa
Purity	>98%
Buffer	Presentation State: Purified State: Lyophilized purified protein. Buffer System: PBS without stabilizer
Preparation	Lyophilized purified protein.
Protein Description	Recombinant Mouse soluble Podoplanin
Storage	The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20°C. Reconstituted soluble Podoplanin should be stored in working aliquots at –20°C. Avoid repeated freeze-thaw cycles.
Endotoxin	< 0.1ng per µg of Mouse soluble Podoplanin
Reference Data
RefSeq	NP_001277751
Locus ID	14726
UniProt ID	Q62011, A8Y5F6
Cytogenetics	4 E1
Synonyms	Gp38; OTS-8; RANDAM-2; T1-alpha; T1a; T1alpha
Summary	Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed:14522983, PubMed:15231832, PubMed:20110424, PubMed:17616532). Interaction with CD9, on the contrary, attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Mediates effects on cell migration and adhesion through its different partners. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (By similarity). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (PubMed:25347465). Through binding with LGALS8 may participate to connection of the lymphatic endothelium to the surrounding extracellular matrix (By similarity). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed:10574709). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (By similarity). Required for normal lung cell proliferation and alveolus formation at birth (PubMed:12654292). Does not function as a water channel or as a regulator of aquaporin-type water channels (By similarity). Does not have any effect on folic acid or amino acid transport (PubMed:12032185).[UniProtKB/Swiss-Prot Function]

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