Placenta growth factor / PGF Rat Protein

Specifications

Product Data
Species	Rat
Expression Host	Insect
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) ALSAGNNSTE MEVVPFNEVW GRSYCRPMEK LVYIADEHPN EVSHIFSPSC VLLSRCSGCC GDEGLHCVAL KTANITMQIL KIPPNRDPHS YVEMTFSQDV LCECRPILET TKAERRKTKG KRKQSKTQTE EPHL Result by N-terminal sequencing: ALSAGNNSTE MEV
Predicted MW	15.14 kDa
Purity	>95% by SDS-PAGE and Silver stain
Buffer	Presentation State: Purified State: Lyophilized freeze dried protein without additives.
Preparation	Lyophilized freeze dried protein without additives.
Protein Description	Recombinant Rat Placenta Growth Factor. The full ORF of rat PlGF (Met1-Leu158) was cloned from total RNA of rat sinusoidal endothelial cells using standard protocols. The native protein expressed in insect cells starts with Ala24.
Storage	The lyophilized Rat PIGF, though stable at room temperature, is best stored in working aliquots at –20°C to –70°C. Avoid repeated freeze-thaw cycles!
Bioactivity	Biological: Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant rat PlGF can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.1-5ng/ml.
Endotoxin	< 0.1ng per µg of Rat PlGF
Reference Data
RefSeq	NP_446047
Locus ID	94203
UniProt ID	Q63434
Cytogenetics	6q31
Synonyms	PGFL, PLGF, PlGF
Summary	forms a heterodimer with VEGF; may play a role in vascular endothelial cell proliferation [RGD, Feb 2006]

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