FKBP2 / FKBP13 (22-142) Human Protein
CAT#: AR09405PU-L
FKBP2 / FKBP13 (22-142) human recombinant protein, 0.25 mg
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CNY 14,920.00
货期*
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规格
Cited in 1 publication. |
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL
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Predicted MW | 13.4 kDa |
Concentration | lot specific |
Purity | >90% by SDS – PAGE |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris buffer (pH 8.0) containing 10% glycerol, 1 mM DTT |
Preparation | Liquid purified protein |
Applications | Protocol: Activity Assay 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2. Add 10 ul of recombinant FKBP2 protein with 1 ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5. Record the increase in A405 nm for 30 minutes at 25°C. |
Protein Description | Recombinant FKBP2 protein was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Bioactivity | Specific: > 270 nmoles/min/mg, defined as the amount of enzyme that cleaves 1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin |
Reference Data | |
RefSeq | NP_001128680 |
Locus ID | 2286 |
UniProt ID | P26885, Q53XJ5 |
Cytogenetics | 11q13.1 |
Synonyms | FKBP-13; FKBP13; PPIase |
Summary | The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008] |
Protein Families | Druggable Genome |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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Ziploc-ing the structure 2.0: Endoplasmic reticulum-resident peptidyl prolyl isomerases show different activities toward hydroxyproline
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The Journal of Biological Chemistry
,PubMed ID 28385890
[FKBP2]
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