GST-Tag (1-224, His-tag) Schistosoma Protein
CAT#: AR09284PU-L
GST-Tag (1-224, His-tag) schistosoma recombinant protein, 0.5 mg
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CNY 13,720.00
货期*
详询
规格
Cited in 2 publications. |
Specifications
Product Data | |
Species | Schistosoma |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
|
Tag | His-tag |
Predicted MW | 28.3 kDa |
Concentration | lot specific |
Purity | >90% by SDS - PAGE |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: PBS, pH 7.4, 10% glycerol |
Preparation | Liquid purified protein |
Protein Description | Recombinant GST, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -80°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Bioactivity | Biological: 2.8-3.3 units/mg, defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C. Activity Assay 1. Prepare a 1 ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25°C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50 ul of recombinant GST protein with various concentrations (1ug, 2ug, 5ug) in 950 ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes. |
Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Reference Data | |
Summary | Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. |
Citations (2)
The use of this Proteins has been cited in the following citations: |
---|
Identification of novel serum autoantibodies against EID3 in non-functional pancreatic neuroendocrine tumors
,null,
Oncotarget
,PubMed ID 29290942
|
Inhibition of pressure-activated cancer cell adhesion by FAK-derived peptides
,null,
Oncotarget
,PubMed ID 29228673
|
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