Streptavidin Protein
CNY 5,210.00
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Specifications
Product Data | |
Description | Streptavidin protein, 1 mg |
Protein Source | S. avidinii |
Concentration | lot specific |
Purity | Prepared from Electrophoretically pure Streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin. No reaction was observed against anti-Avidin. |
Buffer | State: Liquid (sterile filtered) purified Ig fraction. Buffer System: 50 mM Tris Chloride, 150 mM Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 Label: Alkaline Phosphatase (Calf Intestine; Molecular Weight 140, 000 daltons). Preservative: 0.05% (w/v) Sodium Azide Stabilizer: 10 mg/ml BSA (IgG and Protease free) Presentation Label: AP |
Preparation | Liquid (sterile filtered) purified Ig fraction. |
Applications | ELISA: 1/8,000-1/32,000. Western blot: 1/1,000-1/4,000. Immunohistochemistry: 1/200-1/1,000. |
Protein Description | Streptavidin Alkaline Phosphatase Conjugated |
Storage | Store vial at 2-8°C before opening. DO NOT FREEZE! This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
Summary | Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. |
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