C9orf114 (SPOUT1) (NM_016390) Human Mass Spec Standard

Specifications

Product Data
Description	C9orf114 MS Standard C13 and N15-labeled recombinant protein (NP_057474)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC222092
Predicted MW	42.1 kDa
Protein Sequence	Sequence Link (showhide) >RC222092 protein sequence Red=Cloning site Green=Tags(s) MAERGRKRPCGPGEHGQRIEWRKWKQQKKEEKKKWKDLKLMKKLERQRAQEEQAKRLEEEEAAAEKEDRG RPYTLSVALPGSILDNAQSPELRTYLAGQIARACAIFCVDEIVVFDEEGQDAKTVEGEFRGVGKKGQACV QLARILQYLECPQYLRKAFFPKHQDLQFAGLLNPLDSPHHMRQDEESEFREGIVVDRPTRPGHGSFVNCG MKKEVKIDKNLEPGLRVTVRLNQQQHPDCKTYHGKVVSSQDPRTKAGLYWGYTVRLASCLSAVFAEAPFQ DGYDLTIGTSERGSDVASAQLPNFRHALVVFGGLQGLEAGADADPNLEVAEPSVLFDLYVNTCPGQGSRT IRTEEAILISLAALQPGLIQAGARHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_057474
RefSeq Size	4302
RefSeq ORF	1128
Synonyms	C9orf114; CENP-32; CENP32; HSPC109
Locus ID	51490
Cytogenetics	9q34.11
Summary	Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:20813266, PubMed:25657325). Also involved in chromosome alignment (PubMed:20813266). May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis (PubMed:25657325). Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (PubMed:28431233).[UniProtKB/Swiss-Prot Function]
Protein Families	Druggable Genome

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