MRPL34 (NM_023937) Human Mass Spec Standard

Specifications

Product Data
Description	MRPL34 MS Standard C13 and N15-labeled recombinant protein (NP_076426)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC205150
Predicted MW	10.2 kDa
Protein Sequence	Sequence Link (showhide) >RC205150 protein sequence Red=Cloning site Green=Tags(s) MAVLAGSLLGPTSRSAALLGGRWLQPRAWLGFPDAWGLPTPQQARGKARGNEYQPSNIKRKNKHGWVRRL STPAGVQVILRRMLKGRKSLSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_076426
RefSeq Size	968
RefSeq ORF	276
Synonyms	L34mt
Locus ID	64981
Cytogenetics	19p13.11
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

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