URM1 (NM_030914) Human Mass Spec Standard

Specifications

Product Data
Description	URM1 MS Standard C13 and N15-labeled recombinant protein (NP_112176)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200854
Predicted MW	11.4 kDa
Protein Sequence	Sequence Link (showhide) >RC200854 protein sequence Red=Cloning site Green=Tags(s) MAAPLSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVL INDADWELLGELDYQLQDQDSVLFISTLHGG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_112176
RefSeq Size	2650
RefSeq ORF	303
Synonyms	C9orf74
Locus ID	81605
Cytogenetics	9q34.11
Summary	Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.[UniProtKB/Swiss-Prot Function]

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