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Rag2 (NM_009020) Mouse Tagged ORF Clone Lentiviral Particle
CAT＃: MR226630L3V
- LentiORF®
-
Lenti ORF particles, Rag2 (Myc-DDK-tagged) - Mouse recombination activating gene 2 (Rag2), 200ul, >10^7 TU/mL
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CNY 9,785.00
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Specifications
| Product Data | |
| Product Name | Rag2 (NM_009020) Mouse Tagged ORF Clone Lentiviral Particle |
| Synonyms | Rag-2 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| ACCN | NM_009020 |
| ORF Size | 1581 bp |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR226630).
|
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Reference Data | |
| RefSeq | NM_009020.3 |
| RefSeq Size | 3393 bp |
| RefSeq ORF | 1584 bp |
| Locus ID | 19374 |
| Gene Summary | Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.[UniProtKB/Swiss-Prot Function] |
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