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Pold3 (NM_133692) Mouse Tagged ORF Clone Lentiviral Particle
CAT＃: MR207366L3V
- LentiORF®
-
Lenti ORF particles, Pold3 (Myc-DDK-tagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3), 200ul, >10^7 TU/mL
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CNY 9,025.00
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Specifications
| Product Data | |
| Product Name | Pold3 (NM_133692) Mouse Tagged ORF Clone Lentiviral Particle |
| Synonyms | 2410142G14Rik; C85233; P66; P68 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| ACCN | NM_133692 |
| ORF Size | 1386 bp |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR207366).
|
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Reference Data | |
| RefSeq | NM_133692.1, NP_598453.1 |
| RefSeq Size | 3002 bp |
| RefSeq ORF | 1386 bp |
| Locus ID | 67967 |
| Gene Summary | As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:10219083, PubMed:27524497). Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (By similarity).[UniProtKB/Swiss-Prot Function] |
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