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E1 (NC_009824) Virus Tagged ORF Clone
CAT＃: VC102202
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491550
View other clones from "Virus" (8)
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102202 represents NCBI reference of YP_001491550 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGAATGGCGGAACACCAGCGGCTTGTACGTACTCACTAATGATTGCTCTAACTCAAGCATTGTGT ATGAGGCTGACGACGTTATCTTGCACACTCCCGGTTGCGTGCCTTGTGTCCAGGATGGGAACACCTCTAC ATGCTGGACTCCCGTGACCCCTACGGTCGCCGTGCGCTATGTTGGCGCTACTACAGCATCAATCCGAAGT CACGTGGATCTCTTGGTTGGAGCAGCTACTATGTGTTCCGCGTTGTACGTTGGGGACATGTGTGGGGCCG TCTTTCTTGTGGGTCAGGCCTTCACCTTCCGGCCCAGAAGACATCAAACCGTGCAAACCTGCAATTGCTC CCTTTATCCAGGCCACCTGTCCGGGCATAGAATGGCATGGGATATGATGATGAACTGGAGTCCCGCTGTC GGAATGGTCGTTGCGCATGTTCTGCGCCTGCCACAGACACTGTTCGACATCATGGCCGGAGCCCACTGGG GAATTCTCGCAGGTCTGGCATACTACTCAATGCAGGGCAATTGGGCCAAGGTTGCCATCATAATGGTCAT GTTTTCCGGAGTCGATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102202 representing YP_001491550
Red=Cloning sites Green=Tags MLEWRNTSGLYVLTNDCSNSSIVYEADDVILHTPGCVPCVQDGNTSTCWTPVTPTVAVRYVGATTASIRS HVDLLVGAATMCSALYVGDMCGAVFLVGQAFTFRPRRHQTVQTCNCSLYPGHLSGHRMAWDMMMNWSPAV GMVVAHVLRLPQTLFDIMAGAHWGILAGLAYYSMQGNWAKVAIIMVMFSGVDA TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_009824 |
| ORF Size | 579 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_009824.1, YP_001491550 |
| RefSeq ORF | 579 bp |
| MW | 21.0 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102201 | Myc-DDK-tagged ORF clone of viral ORF for C [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491549 |
CNY 3,800.00 |
|
| VC102203 | Myc-DDK-tagged ORF clone of viral ORF for E2/NS1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491551 |
CNY 4,180.00 |
|
| VC102204 | Myc-DDK-tagged ORF clone of viral ORF for NS2 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491552 |
CNY 3,800.00 |
|
| VC102205 | Myc-DDK-tagged ORF clone of viral ORF for NS3 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491553 |
CNY 7,280.00 |
|
| VC102206 | Myc-DDK-tagged ORF clone of viral ORF for NS4a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491554 |
CNY 3,800.00 |
|
| VC102207 | Myc-DDK-tagged ORF clone of viral ORF for NS4b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491555 |
CNY 3,800.00 |
|
| VC102208 | Myc-DDK-tagged ORF clone of viral ORF for NS5a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491556 |
CNY 5,510.00 |
|
| VC102209 | Myc-DDK-tagged ORF clone of viral ORF for NS5b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491557 |
CNY 7,130.00 |
