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gag-pro (NC_001436) Virus Tagged ORF Clone
CAT＃: VC102142
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for Pr gag-pro [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_057861
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CNY 7,272.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102142 represents NCBI reference of NP_057861 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAAATTTTCAGCCGGTCTGCAAGTCCTATTCCTAGACCTCCACGCGGCTTGGCTGCCCATCACT GGTTGAACTTCCTGCAGGCAGCTTATAGGCTGGAGCCAGGCCCTAGTTCTTACGATTTTCACCAGCTGAA AAAGTTCCTCAAGATCGCCCTGGAAACACCAGTTTGGATTTGCCCTATTAACTATTCTCTCCTGGCCTCT CTTCTTCCCAAGGGATATCCCGGCAGAGTGAACGAGATCCTGCACATCCTGATACAGACACAGGCTCAGA TTCCCAGTAGGCCAGCTCCTCCGCCACCCTCTTCCTCCACACATGACCCACCCGATAGCGACCCACAGAT CCCACCGCCGTACGTGGAGCCCACAGCTCCCCAGGTGCTCCCCGTGATGCACCCTCACGGTGCACCTCCA AACCACAGGCCATGGCAGATGAAGGATCTGCAGGCGATCAAACAAGAAGTTAGCCAGGCAGCTCCGGGGT CACCACAATTTATGCAGACGATCCGCCTTGCCGTCCAGCAGTTTGACCCCACAGCCAAGGACCTCCAGGA TTTGCTGCAATACCTTTGCTCTTCTCTCGTCGCTTCTCTGCATCACCAGCAACTTGATAGCCTTATTAGC GAGGCCGAGACCCGGGGCATCACTGGATATAACCCACTCGCGGGTCCCCTCAGAGTCCAGGCCAACAACC CCCAGCAGCAAGGTCTGAGACGCGAATACCAACAGCTCTGGCTTGCTGCCTTTGCCGCTCTGCCAGGAAG CGCCAAAGATCCTTCCTGGGCATCCATCCTGCAGGGACTGGAGGAGCCCTACCACGCCTTTGTCGAAAGA CTGAATATCGCCCTGGATAACGGCCTTCCTGAGGGCACCCCTAAAGATCCCATCCTCAGAAGCCTCGCCT ACAGCAACGCTAATAAAGAATGCCAGAAACTGCTTCAGGCCAGAGGCCATACCAACTCACCGCTCGGCGA CATGCTGAGAGCTTGCCAGGCTTGGACCCCTAAAGACAAAACCAAGGTCCTGGTAGTTCAGCCAAAAAAG CCCCCTCCAAACCAGCCCTGCTTCAGGTGCGGGAAAGCCGGGCATTGGTCTCGGGACTGTACCCAACCCA GGCCGCCTCCTGGCCCATGTCCTCTCTGCCAAGACCCAACGCACTGGAAACGGGATTGCCCCCGACTTAA GCCTACCATCCCCGAGCCCGAACCCGAAGAGGACGCCCTTCTGCTTGACCTTCCTGCCGACATCCCGCAC CCTAAGAATCTTCACAGGGGAGGAGGCCTTACATCTCCCCCAACTTTGCAACAGGTGCTGCCGAATCAGG ATCCAACATCTATCCTCCCGGTTATACCACTTGATCCAGCGAGAAGACCCGTGATTAAAGCACAGATTGA CACACAGACCAGCCACCCCAAAACAATAGAGGCCTTGCTGGACACCGGGGCCGATATGACGGTGCTCCCA ATTGCTCTGTTTAGTAGTAATACCCCACTGAAGAACACCAGCGTTCTGGGCGCAGGCGGCCAGACCCAGG ACCACTTCAAATTGACCTCCCTCCCTGTGCTGATAAGGTTGCCGTTTAGAACCACTCCTATTGTTCTTAC CTCATGTCTCGTCGACACCAAGAACAACTGGGCGATTATTGGGCGAGACGCTCTGCAACAGTGTCAGGGG GTCCTGTACCTCCCCGAGGCTAAACGACCCCCCGTTATATTGCCTATCCAGGCCCCCGCGGTCCTGGGGC TTGAACACCTTCCTAGACCTCCCGAGATTAGCCAGTTTCCCCTGAATCAGAATGCCTCCAGACCATGTAA CACGTGGAGCGGCCGCCCATGGAGACAAGCGATTAGCAATCCCACACCAGGGCAAGAGATAACACAATAC AGCCAGCTGAAGAAGCCTATGGAGCCTGGAGATAGTTCAACCACTTGCGGGCCTCTGACACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102142 representing NP_057861
Red=Cloning sites Green=Tags MGQIFSRSASPIPRPPRGLAAHHWLNFLQAAYRLEPGPSSYDFHQLKKFLKIALETPVWICPINYSLLAS LLPKGYPGRVNEILHILIQTQAQIPSRPAPPPPSSSTHDPPDSDPQIPPPYVEPTAPQVLPVMHPHGAPP NHRPWQMKDLQAIKQEVSQAAPGSPQFMQTIRLAVQQFDPTAKDLQDLLQYLCSSLVASLHHQQLDSLIS EAETRGITGYNPLAGPLRVQANNPQQQGLRREYQQLWLAAFAALPGSAKDPSWASILQGLEEPYHAFVER LNIALDNGLPEGTPKDPILRSLAYSNANKECQKLLQARGHTNSPLGDMLRACQAWTPKDKTKVLVVQPKK PPPNQPCFRCGKAGHWSRDCTQPRPPPGPCPLCQDPTHWKRDCPRLKPTIPEPEPEEDALLLDLPADIPH PKNLHRGGGLTSPPTLQQVLPNQDPTSILPVIPLDPARRPVIKAQIDTQTSHPKTIEALLDTGADMTVLP IALFSSNTPLKNTSVLGAGGQTQDHFKLTSLPVLIRLPFRTTPIVLTSCLVDTKNNWAIIGRDALQQCQG VLYLPEAKRPPVILPIQAPAVLGLEHLPRPPEISQFPLNQNASRPCNTWSGRPWRQAISNPTPGQEITQY SQLKKPMEPGDSSTTCGPLTL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001436 |
| ORF Size | 1953 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001436.1, NP_057861 |
| RefSeq ORF | 1953 bp |
| MW | 71.7 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102141 | Myc-DDK-tagged ORF clone of viral ORF for Pr55 [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_057862 |
CNY 5,230.00 |
|
| VC102144 | Myc-DDK-tagged ORF clone of viral ORF for gp46 SU [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_057865 |
CNY 5,990.00 |
|
| VC102145 | Myc-DDK-tagged ORF clone of viral ORF for p40 [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_057864 |
CNY 5,488.00 |
|
| VC102146 | Myc-DDK-tagged ORF clone of viral ORF for p27 [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_057863 |
CNY 4,470.00 |
|
| VC102147 | Myc-DDK-tagged ORF clone of viral ORF for p19 MA [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_955617 |
CNY 3,800.00 |
|
| VC102148 | Myc-DDK-tagged ORF clone of viral ORF for p24 CA [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_955618 |
CNY 3,800.00 |
|
| VC102150 | Myc-DDK-tagged ORF clone of viral ORF for gp46 SU [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_955620 |
CNY 5,990.00 |
|
| VC102151 | Myc-DDK-tagged ORF clone of viral ORF for p21 TM [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_955621 |
CNY 3,800.00 |
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