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VP2 polypeptide (NC_001617) Virus Tagged ORF Clone
CAT＃: VC102119
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for VP2 polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740392
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CNY 3,800.00
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CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102119 represents NCBI reference of NP_740392 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCCACAGTGGAGGCTTGTGGATACTCCGATAGACTGATTCAAATTACCCGGGGGGATTCAACTA TCACATCTCAGGATACTGCTAATGCAGTGGTCGCGTATGGTGTGTGGCCTAGCTACCTTACTCCTGACGA TGCCACAGCTATCGACAAACCTACACAACCTGACACATCTAGTAATAGGTTCTACACCCTGGACTCCCGG TCCTGGACATCAGCTTCCTCTGGATGGTGGTGGAAGCTCCCTGATGCTCTGAAGAATATGGGAATTTTCG GAGAGAACATGTTCTACCACTTCCTGGGGAGATCAGGATATACAATCCACGTGCAGTGTAACAGCTCAAA ATTCCACCAGGGGCTGCTTATAGTCGCAGCCATCCCAGAACACCAACTTGCTTCAGCGACGAGCGGGAAC GTGTCAGTCGGGTATAATCACACGCACCCCGGCGAACAGGGCCGGGAAGTAGTGCCATCACGCACCTCTT CAGATAACAAGCGACCTAGCGATGATAGTTGGCTGAATTTCGACGGTACACTGCTGGGTAATCTGCCAAT CTATCCCCACCAATACATAAATCTGCGAACGAACAACTCCGCCACACTGATTTTGCCCTATGTTAACGCC GTGCCGATGGACAGCATGTTGAGACACAATAATTGGAGCCTGGTGATCATCCCCATATGCCCACTGCAGG TTCAGCCTGGAGGGACTCAGTCAATCCCGATCACTGTCTCTATCAGTCCTATGTTTTCCGAATTTAGCGG ACCACGATCCAAGGTCGTTTTTTCAACTACACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102119 representing NP_740392
Red=Cloning sites Green=Tags MSPTVEACGYSDRLIQITRGDSTITSQDTANAVVAYGVWPSYLTPDDATAIDKPTQPDTSSNRFYTLDSR SWTSASSGWWWKLPDALKNMGIFGENMFYHFLGRSGYTIHVQCNSSKFHQGLLIVAAIPEHQLASATSGN VSVGYNHTHPGEQGREVVPSRTSSDNKRPSDDSWLNFDGTLLGNLPIYPHQYINLRTNNSATLILPYVNA VPMDSMLRHNNWSLVIIPICPLQVQPGGTQSIPITVSISPMFSEFSGPRSKVVFSTTQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001617 |
| ORF Size | 804 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001617.1, NP_740392 |
| RefSeq ORF | 804 bp |
| MW | 29.5 kDa |
Documents
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102118 | Myc-DDK-tagged ORF clone of viral ORF for VP4 polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740391 |
CNY 3,800.00 |
|
| VC102120 | Myc-DDK-tagged ORF clone of viral ORF for VP3 polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740393 |
CNY 3,800.00 |
|
| VC102121 | Myc-DDK-tagged ORF clone of viral ORF for VP1 polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740394 |
CNY 3,800.00 |
|
| VC102122 | Myc-DDK-tagged ORF clone of viral ORF for P2-A polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740395 |
CNY 3,800.00 |
|
| VC102123 | Myc-DDK-tagged ORF clone of viral ORF for P2-B polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740396 |
CNY 3,800.00 |
|
| VC102124 | Myc-DDK-tagged ORF clone of viral ORF for P2-C polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740397 |
CNY 3,800.00 |
|
| VC102125 | Myc-DDK-tagged ORF clone of viral ORF for P-3A polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740398 |
CNY 3,800.00 |
|
| VC102126 | Myc-DDK-tagged ORF clone of viral ORF for VPg polypeptide [Human rhinovirus A], codon optimized for human cell expression, NP_740399 |
CNY 3,800.00 |
|
| VC102127 | Myc-DDK-tagged ORF clone of viral ORF for protease [Human rhinovirus A], codon optimized for human cell expression, NP_740400 |
CNY 3,800.00 |
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