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3C (protease) (NC_001490) Virus Tagged ORF Clone
CAT＃: VC102115
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for 3C (protease) [Human rhinovirus 14], codon optimized for human cell expression, NP_740524
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CNY 3,600.00
| Cited in 1 publication. |
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102115 represents NCBI reference of NP_740524 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCGAATACAGAATTCGCCTTGTCATTGCTGAGGAAAAACATTATGACCATCACGACATCTAAGG GAGAATTTACTGGGTTGGGCATTCATGACAGAGTCTGTGTGATCCCTACCCACGCCCAACCCGGAGACGA CGTGTTGGTGAATGGCCAAAAGATTCGCGTGAAAGACAAGTATAAGTTGGTCGATCCAGAGAATATCAAT CTGGAACTCACCGTGCTGACACTGGATAGAAACGAGAAGTTCCGAGATATCCGAGGTTTTATCAGTGAGG ATCTCGAGGGGGTAGACGCGACTCTGGTGGTGCACAGCAATAACTTTACTAATACGATTTTGGAGGTGGG GCCTGTTACTATGGCTGGCCTGATAAACCTGAGCAGCACTCCCACGAATCGCATGATCAGGTACGATTAT GCTACTAAGACCGGACAATGTGGTGGCGTCCTGTGTGCCACCGGGAAGATCTTCGGGATCCATGTAGGCG GGAACGGAAGGCAGGGCTTTAGCGCACAACTGAAGAAGCAGTATTTCGTGGAAAAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102115 representing NP_740524
Red=Cloning sites Green=Tags MGPNTEFALSLLRKNIMTITTSKGEFTGLGIHDRVCVIPTHAQPGDDVLVNGQKIRVKDKYKLVDPENIN LELTVLTLDRNEKFRDIRGFISEDLEGVDATLVVHSNNFTNTILEVGPVTMAGLINLSSTPTNRMIRYDY ATKTGQCGGVLCATGKIFGIHVGGNGRQGFSAQLKKQYFVEKQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001490 |
| ORF Size | 549 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001490.1, NP_740524 |
| RefSeq ORF | 549 bp |
| MW | 20.1 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Intracellular sensing of complement C3 activates cell autonomous immunity
,null,
Science (New York, N.Y.)
,PubMed ID 25190799
[3C (protease)]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102106 | Myc-DDK-tagged ORF clone of viral ORF for 1A (VP4) [Human rhinovirus 14], codon optimized for human cell expression, NP_740515 |
CNY 3,800.00 |
|
| VC102107 | Myc-DDK-tagged ORF clone of viral ORF for 1B (VP2) [Human rhinovirus 14], codon optimized for human cell expression, NP_740516 |
CNY 2,640.00 |
|
| VC102108 | Myc-DDK-tagged ORF clone of viral ORF for 1C (VP3) [Human rhinovirus 14], codon optimized for human cell expression, NP_740517 |
CNY 2,640.00 |
|
| VC102109 | Myc-DDK-tagged ORF clone of viral ORF for 1D (VP1) [Human rhinovirus 14], codon optimized for human cell expression, NP_740518 |
CNY 3,600.00 |
|
| VC102110 | Myc-DDK-tagged ORF clone of viral ORF for 2A (P2-A) [Human rhinovirus 14], codon optimized for human cell expression, NP_740519 |
CNY 1,800.00 |
|
| VC102111 | Myc-DDK-tagged ORF clone of viral ORF for 2B (P2-B) [Human rhinovirus 14], codon optimized for human cell expression, NP_740520 |
CNY 1,200.00 |
|
| VC102112 | Myc-DDK-tagged ORF clone of viral ORF for 2C (P2-C) [Human rhinovirus 14], codon optimized for human cell expression, NP_740521 |
CNY 2,400.00 |
|
| VC102113 | Myc-DDK-tagged ORF clone of viral ORF for 3A (P3-A) [Human rhinovirus 14], codon optimized for human cell expression, NP_740522 |
CNY 1,200.00 |
|
| VC102114 | Myc-DDK-tagged ORF clone of viral ORF for 3B (VPg) [Human rhinovirus 14], codon optimized for human cell expression, NP_740523 |
CNY 3,800.00 |
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