HN (NC_003443) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102056 represents NCBI reference of NP_598405 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTACTCTAACCTCTCACTGAAGTCCATCCCCAAGCGCACCTGCCGAATAATTTTCCGCACTG CAACAATACTGGGCATTTGCACCCTGATCGTGCTGTGCAGCAGCATACTGCATGAGATTATACACCTCGA CGTGTCATCCGGCCTGATGGACTCTGATGACTCACAACAAGGAATTATCCAGCCCATTATTGAAAGCTTG AAGTCACTGATTGCCCTTGCCAATCAGATCCTTTACAACGTGGCAATCATTATCCCTCTGAAGATCGACT CTATCGAAACAGTTATTTTCTCTGCACTGAAGGATATGCACACCGGTAGCATGAGCAATACCAACTGTAC CCCCGGCAACCTTCTGCTGCACGATGCTGCCTATATAAACGGAATCAATAAATTTCTGGTCCTGAAGTCA TACAACGGCACCCCAAAATATGGCCCCCTGTTGAACATCCCCTCCTTCATCCCTTCTGCCACAAGTCCTA ATGGCTGTACGAGAATTCCAAGCTTCTCTCTGATTAAGACACATTGGTGCTACACACATAATGTTATGTT GGGCGACTGCCTGGACTTCACCACATCTAACCAATACCTGGCCATGGGAATCATCCAGCAGAGTGCAGCG GCATTTCCAATCTTCAGGACTATGAAAACCATTTATCTTTCCGATGGCATCAATCGCAAGTCCTGCAGCG TAACCGCAATTCCGGGCGGGTGTGTTCTCTACTGCTACGTCGCCACCAGATCAGAGAAGGAGGACTATGC CACCACCGACCTTGCAGAGCTGAGATTGGCGTTTTATTACTATAACGATACCTTTATCGAGAGGGTCATC TCACTTCCAAACACAACTGGGCAGTGGGCCACCATTAATCCCGCTGTGGGTTCCGGAATATATCACCTGG GCTTCATTCTTTTTCCTGTCTATGGGGGCCTCATAAGTGGGACCCCATCCTATAATAAGCAGAGTTCAAG ATATTTCATACCAAAGCACCCAAACATCACGTGCGCCGGAAATTCTTCTGAACAGGCTGCTGCGGCTCGA TCTAGCTACGTGATACGGTATCATAGTAACCGACTGATTCAGTCTGCCGTCCTGATATGTCCACTTTCCG ATATGCACACGGCCCGCTGCAACCTGGTGATGTTTAACAATTCCCAGGTTATGATGGGAGCCGAGGGTAG GTTGTACGTAATTGACAACAACCTGTATTACTACCAGAGGTCCAGTTCCTGGTGGTCCGCGAGTCTCTTT TATAGGATCAATACAGACTTCTCCAAGGGTATTCCTCCAATCATCGAGGCCCAGTGGGTGCCATCTTATC AAGTGCCCCGGCCCGGCGTTATGCCATGTAACGCCACTAGTTTCTGCCCTGCCAATTGTATCACCGGAGT ATACGCGGACGTCTGGCCCCTTAACGATCCCGAGCCAACCAGTCAGAACGCCCTCAATCCGAACTATAGG TTTGCCGGTGCATTCCTGAGGAATGAAAGCAACAGAACGAACCCTACATTTTACACAGCCTCTGCATCCG CACTGCTGAACACGACCGGTTTTAATAACACAAATCATAAGGCAGCCTACACGAGCTCCACGTGTTTTAA GAATACAGGGACTCAGAAGATCTACTGCCTGATCATAATCGAAATGGGAAGCAGCCTGCTGGGCGAATTT CAGATCATTCCCTTTCTGAGAGAGCTGATCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102056 representing NP_598405 Red=Cloning sites Green=Tags MEDYSNLSLKSIPKRTCRIIFRTATILGICTLIVLCSSILHEIIHLDVSSGLMDSDDSQQGIIQPIIESL KSLIALANQILYNVAIIIPLKIDSIETVIFSALKDMHTGSMSNTNCTPGNLLLHDAAYINGINKFLVLKS YNGTPKYGPLLNIPSFIPSATSPNGCTRIPSFSLIKTHWCYTHNVMLGDCLDFTTSNQYLAMGIIQQSAA AFPIFRTMKTIYLSDGINRKSCSVTAIPGGCVLYCYVATRSEKEDYATTDLAELRLAFYYYNDTFIERVI SLPNTTGQWATINPAVGSGIYHLGFILFPVYGGLISGTPSYNKQSSRYFIPKHPNITCAGNSSEQAAAAR SSYVIRYHSNRLIQSAVLICPLSDMHTARCNLVMFNNSQVMMGAEGRLYVIDNNLYYYQRSSSWWSASLF YRINTDFSKGIPPIIEAQWVPSYQVPRPGVMPCNATSFCPANCITGVYADVWPLNDPEPTSQNALNPNYR FAGAFLRNESNRTNPTFYTASASALLNTTGFNNTNHKAAYTSSTCFKNTGTQKIYCLIIIEMGSSLLGEF QIIPFLRELIP TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_003443
ORF Size	1713 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_003443.1, NP_598405
RefSeq ORF	1713 bp
Locus ID	935188
MW	63.3 kDa

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