E6 (NC_001596) Virus Tagged ORF Clone

CAT＃: VC102014

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041860

Datasheet

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CNY 3,800.00

货期*

4周

规格

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102014 represents NCBI reference of NP_041860 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCTCACTGAGCAGATTATGGATCGCCCGAAACCAAGGACCGTGAAGGAACTGGCTGATACTCTCG TTATTCCTCTGATAGACCTCCTGATCCCTTGTAAATTTTGTAACAGATTTCTCTCTTACTTTGAACTGTT GAACTTCGATCATAAATGCCTGCAGCTTATCTGGACCGAGGAGGATTTGGTCTACGGCTTGTGTTCCTCC TGCGCATACGCATCCGCCCAGCTGGAATTCACTCACTTCTTCCAGTTTGCCGTGGTGGGCAAGGACATTG AAACAGTGGAAGGCACTGCTATAGGGAACATCTGTATAAGATGTAGATATTGTTTTAAACTGCTGGATCT GGTTGAGAAATTGGCAACCTGTTACAAATTCGAGCAGTTCTATAAAGTTCGCAACAGTTGGAAGGGCCTT TGCCGCCACTGCGGAAGTGTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102014 representing NP_041860 Red=Cloning sites Green=Tags MYLTEQIMDRPKPRTVKELADTLVIPLIDLLIPCKFCNRFLSYFELLNFDHKCLQLIWTEEDLVYGLCSS CAYASAQLEFTHFFQFAVVGKDIETVEGTAIGNICIRCRYCFKLLDLVEKLATCYKFEQFYKVRNSWKGL CRHCGSVE TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_001596
ORF Size	444 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001596.1, NP_041860
RefSeq ORF	444 bp
Locus ID	1489480
MW	17.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC102015	Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041861	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102016	Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041862	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC102017	Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041863	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,320.00
VC102018	Myc-DDK-tagged ORF clone of viral ORF for early protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041864	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,610.00
VC102019	Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041865	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
VC102020	Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 9], codon optimized for human cell expression, NP_041866	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,180.00

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