E1 (NC_012213) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101846 represents NCBI reference of YP_002647035 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTGGCCACGCACTAAGGAGTCTGAGGGCCTTTCCAACAGCTGCGTCGAAACTCTTACCCTCTGTG TGCTTCCAGTGCCCAGCAATGAGAATGAGATGGAGGACAAGGATACAAAGGGTACGTCCTTCTGTATCGA TAACACTACCGAAGCCATTCAGTTCATCGAAACCGAAGCAGAATGCGACGAATGTCTTGATAGTCTGGAG GCACTGTTCGAGGAATCAGACGGGACTGATGTGTCCGACCTCATCAACAATATCGACGAGCCCGACGAGG GAAACCCCCACGCCCTCCTGAACAGGCAGCAGCTGGAGGAGGATTCACAACTGCTGAGCGTGCTGAAGAG GAAATATACCTCCCCCTCCCCCAAGCAGCAGGTGCTCGACCTTTCCCCCAGGCTGGAGTCACTCTGTGTG TCCCCAAGGGGCACCTCTTCAAAGCGAAGGCTTTTTGAAGGTTCAGGGCTTGGGCACGAAGCAGAGGACT CTAACGAAAGAGTGGAGAAGGTCGCGTCCCAGGCCGCACAGCCTAGCAACGAAACGAACGGCGAGCAGGA ACAGCAAAGTGTTTCAGCCACACAGAATGGCGACACCCAGTGCCTTGATATTCTTCACTCAAACAACCGG TTGGCCACCGCCCTGACAAAATTCAAGAATACCTTTGGAGTGGGATACAAGGAACTCACCAGGCCCTTCA AATCAAACAAGACTTGCTGCCACTCTTGGGTGGCTGTGATCTATGGGGTGCGAGCGGAAATGCTCGAGGC TAGCAAGACTCTGTTGAAGCCCCATTGTGACTTCTTTCAAATCCTGAACCCGAGCATGGGAACAGGAGTG ACCGTGCTGATCCTGTTTGAATTTGCCAGCTCAAAGTGTAGGGACACCGTCACAAGGTTGCTCTGTAATA TCTTCAACGTTGAAGAGTACCAAATCCTCGCAGATCCACCTAGACACAAAAGCGTCGCCGTCGCCCTGTT TTTTTACAGGCACTCACTGTCCAACATAAGCTTCACCGAAGGTGTCATGCCAGATTGGCTCAAGAAGCAA ACTATGCTGAATCATCAGCAGGAAGCGGACACGTTTGAACTGGCACCAATGGTCCAATGGGCCTACGACA ATAACATCTGGGAGGAAGCCGAGCTGGCCTATCAGTATGCGTGCTTGGCAGACGTCGAACCTAACGCAGC TGCTTGGCTGAAGTCCAATAACCAGTACAAGTATGTGTCCGATTGTGCAAAAATGGTAAAGATGTATAAG CGGTACGAGATGAGACAGATGAGTATAGGCCAGTGGATCAAGAAGTGCGGCGAGACGGTCGATGGCGAGG GAGATTGGAAAAAACTGATCAATCTCCTGAAGTACCAGGACGTCTCAATCATTCCATTCCTGACCAGCCT GAGGCTGTTCTTGAAAGGCGAGCCGAAAAAGAACTGCATCGTGATCTGGGGTCCTCCTGACACCGGGAAA TCCATGTTCTGCTACTCCTTGATTCGGTATCTTCAGGGGAAGGTGGTCTCATATGCCAACTCTAAAAGTC AGTTCTGGCTCCAGCCTCTTACGGAGGCCAAAATTGGTCTGATCGATGACGCAACGTTTCCATGTTTCCA GTTCATGGATGTATACATGAGGTCCGGACTCGATGGGAACGAAGTCTCCGTTGATTGTAAACATAAGACC CCCATGCAAGTAAAGTTGCCGCCTATGTTGGTGACCAGTAATATCAATGTTCACGCAGAGACTTCCCTGA AGTACCTGCACAGCAGACTCACCGGATACAATTTTCCCCATAAGCTCCCTGTAGACTCTAACGGAAACGT GGTGTATAAGATTACACACGCAGATTGGAAATGCTTTCTCGCCTCATGGTCCTCAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101846 representing YP_002647035 Red=Cloning sites Green=Tags MCWPRTKESEGLSNSCVETLTLCVLPVPSNENEMEDKDTKGTSFCIDNTTEAIQFIETEAECDECLDSLE ALFEESDGTDVSDLINNIDEPDEGNPHALLNRQQLEEDSQLLSVLKRKYTSPSPKQQVLDLSPRLESLCV SPRGTSSKRRLFEGSGLGHEAEDSNERVEKVASQAAQPSNETNGEQEQQSVSATQNGDTQCLDILHSNNR LATALTKFKNTFGVGYKELTRPFKSNKTCCHSWVAVIYGVRAEMLEASKTLLKPHCDFFQILNPSMGTGV TVLILFEFASSKCRDTVTRLLCNIFNVEEYQILADPPRHKSVAVALFFYRHSLSNISFTEGVMPDWLKKQ TMLNHQQEADTFELAPMVQWAYDNNIWEEAELAYQYACLADVEPNAAAWLKSNNQYKYVSDCAKMVKMYK RYEMRQMSIGQWIKKCGETVDGEGDWKKLINLLKYQDVSIIPFLTSLRLFLKGEPKKNCIVIWGPPDTGK SMFCYSLIRYLQGKVVSYANSKSQFWLQPLTEAKIGLIDDATFPCFQFMDVYMRSGLDGNEVSVDCKHKT PMQVKLPPMLVTSNINVHAETSLKYLHSRLTGYNFPHKLPVDSNGNVVYKITHADWKCFLASWSSS TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_012213
ORF Size	1878 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_012213.1, YP_002647035
RefSeq ORF	1878 bp
Locus ID	7559098
MW	70.8 kDa

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