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E1 (NC_001576) Virus Tagged ORF Clone
CAT＃: VC101834
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041743
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CNY 10,260.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101834 represents NCBI reference of NP_041743 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGATAATACCGGGACCGAAGGCGGAGCCTGTTCAGAGTCAGAAAGGGCAGGAGGCTGGTTTATCG TGGAGGCTATTGTGGATAGAAGGACTGGCGATCCCATCAGTTCTGATGACGACGAGGAGGAGGACGAGGC TGGAGAGGACTTCGTGGACTTCATCGATGACACCAGGTCCCTGGGAGACGGCCAAGAGGTGGCACAAGAG CTCTTCCAGCAGCAAACCGCTGCAGATGACGATGTGGCAGTGCAGACAGTGAAGAGGAAGTTTGCCCCAT CCCCATACTTCAGCCCCGTGTGCGAACAGGCGAGCATCGAACATGAGCTGTCTCCCCGGCTGGATGCTAT TAAACTCGGGCGCCAATCTGCGAAGGCGAAACGACGGCTCTTCGAGCTGCCCGATAGCGGGTATGGTCAG ACACAGGTAGACACTGAATCCGGACCTAAGCAAGTACAGGGCTCTAGTGAAACACAGGATGGCAGACAGG ATGATGATGAGGGGTCTGTAGTTCAGTCTACCCTCGACACAGGGAACCAGAACGGTCGGCAAAACAACGA CGAGGGATCTGGACGCAATGTGGGTGAACATGGTAGCCAGGAAGAGGAGAGGGCCGGGGGCGACGGAGAA GAGAGTGACCTGCAGTCTACATCCACAGGGAAGGGTGCAGGAGGAGTAGTTGAGATTCTGAGGGCAAGCA ATAAAAAGGCAACACTTCTTGGCAAATTCAAAGAACAGTTCGGCCTGGGCTACAACGAACTTATTCGGCA TTTTAAAAGCGACCGAACGTCATGCGCCGACTGGGTGGTCTGTGTTTTCGGCGTCTTCTGCACAGTCGCT GAAGGCATCAAGACTCTCATTCAGCCTCTTTGCGACTACGCTCATATCCAGGTGCTGCCCTGCCAGTGGG GCATGACAGTGTTGATGCTGGTTAGGTACAAGCGGGCCAAAAACAGAGAAACAGTGGCAAAAGGACTGAG TACACTTCTGAATGTGCCGGAGAGCCAAATGCTTATTGAGCCTCCCAAATTGCGGTCTGGGCCAGCCGCC CTGTATTGGTATAAAACTTCTATGTCATCCTGTTCAGACGTGTACGGCGAGACCCCCGAGTGGATTGTGA GGCAGACTATGGTCGGTCACGCCATGGAGGATGCGCAGTTTAGCCTGTCAGAAATGGTCCAGTGGGCATA CGACCACGACATTACAGATGAAAGCACCCTGGCATACGAGTATGCTCTGATCGCCGATACAGATTCTAAT GCCGCCGCTTTCTTGTCATCAAATTGCCAGGCCAAATATCTGAAGGATGCCTGTACAATGTGTAGACATT ACAAACGGGGCGAGCAGGCCCGCATGAGCATGTCTGAGTGGATCTGGTTTAGAGGCGACAAAGTACAGGG CGATGGAGATTGGAAACCCATAGTTCAATTTTTGCGATATCAGGATGTTGAATTCATACCTTTTCTGTGC GCCTTCAAAACCTTTCTGCAGGGAGTGCCCAAGAAGAGCTGCTTGGTGTTCTATGGGCCTGCCGACACTG GCAAGAGCTATTTCTGCATGAGTCTGCTGCGGTTTCTCGGAGGGGCAGTTATTAGCTATGCCAATTCTTC CTCCCATTTTTGGCTGCAGCCACTCAGCGAGGCCAAAATTGGTTTGTTGGACGATGCTACCTCTCAGTGC TGGAATTACATCGACACATACCTCCGAAACGCTCTCGACGGGAACCAGATTTGTGTGGACAGAAAACATC GAGCCTTGCTGCAGTTGAAATGTCCTCCTCTGCTTATCACTACTAACATCAACCCTCTCACCGATGAAAG ATGGAAATTCCTGAGGTCTCGCTTGCAACTTTTCACATTCAAGAATCCATTCCCAGTTACAACTCAGGGC GAACCTATGTATACCCTGAATGACCAGAACTGGAAGTGCTTCTTCAGGCGGCTGTGGGCCAGGCTGAGTC TCACCGATCCCGAAGACGAGGAGGAGCACGGCAATCCCTCCGAGCCTTTCCGCTGCGTGCCCGGACAGAA TGCTAGGACCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101834 representing NP_041743
Red=Cloning sites Green=Tags MDDNTGTEGGACSESERAGGWFIVEAIVDRRTGDPISSDDDEEEDEAGEDFVDFIDDTRSLGDGQEVAQE LFQQQTAADDDVAVQTVKRKFAPSPYFSPVCEQASIEHELSPRLDAIKLGRQSAKAKRRLFELPDSGYGQ TQVDTESGPKQVQGSSETQDGRQDDDEGSVVQSTLDTGNQNGRQNNDEGSGRNVGEHGSQEEERAGGDGE ESDLQSTSTGKGAGGVVEILRASNKKATLLGKFKEQFGLGYNELIRHFKSDRTSCADWVVCVFGVFCTVA EGIKTLIQPLCDYAHIQVLPCQWGMTVLMLVRYKRAKNRETVAKGLSTLLNVPESQMLIEPPKLRSGPAA LYWYKTSMSSCSDVYGETPEWIVRQTMVGHAMEDAQFSLSEMVQWAYDHDITDESTLAYEYALIADTDSN AAAFLSSNCQAKYLKDACTMCRHYKRGEQARMSMSEWIWFRGDKVQGDGDWKPIVQFLRYQDVEFIPFLC AFKTFLQGVPKKSCLVFYGPADTGKSYFCMSLLRFLGGAVISYANSSSHFWLQPLSEAKIGLLDDATSQC WNYIDTYLRNALDGNQICVDRKHRALLQLKCPPLLITTNINPLTDERWKFLRSRLQLFTFKNPFPVTTQG EPMYTLNDQNWKCFFRRLWARLSLTDPEDEEEHGNPSEPFRCVPGQNARTI TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001576 |
| ORF Size | 2043 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001576.1, NP_041743 |
| RefSeq ORF | 2043 bp |
| Locus ID | 1489373 |
| MW | 76.4 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101832 | Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041741 |
CNY 3,800.00 |
|
| VC101833 | Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041742 |
CNY 3,800.00 |
|
| VC101835 | Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041744 |
CNY 4,470.00 |
|
| VC101836 | Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041745 |
CNY 5,700.00 |
|
| VC101837 | Myc-DDK-tagged ORF clone of viral ORF for late protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041746 |
CNY 6,460.00 |
|
| VC101838 | Myc-DDK-tagged ORF clone of viral ORF for late protein, partial [Human papillomavirus type 10], codon optimized for human cell expression, NP_041747 |
CNY 6,080.00 |
