E1 (NC_001352) Virus Tagged ORF Clone

CAT#: VC101813

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for early protein E1 [Alphapapillomavirus 4], codon optimized for human cell expression, NP_077118



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CNY 7,700.00


货期*
5周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101813 represents NCBI reference of NP_077118 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGACTCAGAGGGGACCGACGGTACCGAGGAGGATGGTTGCCGGGCGGGGGGCTGGTTTCACGTTG
AAGCTATCATTACCCACGGTCAGCGGCAGGTATCATCAGATGAAGACGAGGACGAGACCGAGACCGGCGA
GGACCTCGACTTCATCGATAATCGCGTTCCCGGTGATGGACAAGAGATTCCTCTGCAGCTGTACGCACAA
CAAACTGCCCAGGATGATGAAGCCACAGTGCAGGCCTTGAAGCGCAAGTTCGTCGCTTCCCCTTTGTCCG
CCTGCTCTTGCATCGAAAACGACCTGTCCCCCCGGCTGGACGCCATCAGTCTCAATCGGAAATCTGAGAA
GGCTAAGCGCAGGCTTTTCGAGACCGAGCCTCCCGACAGTGGATATGGCAATACACAAATGGTGGTGGGG
ACACCGGAAGAGGTCACCGGCGACGAGGAATCTCAGGGTGGGAGACCTGTGGAAGACCAGGAAGAGGAGC
GCCAAGGCGGCGATGGCGAGGCTGATCTCACAGTGCATACACCGCAGTCTGGCACAGATGCAGCTGGGTC
TGTCCTGACCTTGTTGCGAAGTAGCAACCTGAAAGCGACACTTCTGAGCAAATTTAAAGACCTGTTTGGC
GTAGGCTTTTACGAACTCGTGCGGCAGTTCAAAAGCTCTAAGACCGCCTGCGCCGATTGGGTAGTGTGCG
CCTACGGGGTATATTACGCGGTCGCTGAAGGACTCAAGAAACTGATCCAACCACACACCCAGTACGCCCA
TATCCAGGTGCAGACCTCTTCTTGGGGCATGGTCGTTTTCATGCTCCTCCGCTATAACTGCGCAAAGAAC
CGAGACAGTGTCTCCAAAAATATGAGCATGCTTTTGAACATTCCTGAGAAGCATATGTTGATAGAGCCCC
CTAAGCTGCGCAGCACACCGGCTGCGCTTTACTGGTACAAAACAGCCATGGGAAATGGTTCCGAGGTGTA
TGGCGAAACACCTGAATGGATCGTGCGGCAAACCCTTGTGGGACATTCAATGGAGGATGAACAGTTCAGG
CTGAGTGTGATGGTGCAATATGCATACGACCACGACATTGTCGAGGAAAGCGTGCTCGCATTCGAGTACG
CACAGCTGGCCGATGTAGATGCCAATGCGGCTGCATTCCTGAATTCTAACTGCCAGGCTAAGTATGTGAA
AGATGCTGTCACCATGTGCCGACATTACAAGAGGGCCGAGAGGGAGCAGATGTCTATGTCACAGTGGATT
ACCTTTAGGGGAAATAAGGTGTCTGAAGAGGGGGATTGGAAGCCAATTGTTAGATTCCTGCGGCACCAAG
GCGTGGAGTTCGTCAGCTTCCTGGCTGCATTTAAACTGTTCTTGAAAGGAGTCCCGAAAAAGAATTGTAT
CGTTTTTTATGGACCTGCCGATACAGGGAAGTCATACTTTTGCATGTCCCTCTTGCAATTTCTTGGGGGG
GCTGTTATCTCATACGCTAACTCAAGTTCACACTTTTGGCTTCAGCCTTTGAGTGACTCCAAGATTGGGC
TCCTGGACGACGCCACCCCACAGTGCTGGTCATATATTGACATCTACCTTAGGAACCTGCTCGACGGCCA
CCCCGTTAGCATTGATAGAAAGCACAAAACTCTCCTGCAGCTCAAGTGTCCCCCCCTCATGATCACCACA
AACACAAACCCTCTGGAAGAGGACCGCTGGAAGTACCTCCGGAGTAGACTTACAGTTTTCACATTTAAGA
ATCCTTTCCCATTCGCTTCCCCCGGCGAGCCGCTGTACCCGATCAATAATGCCAATTGGAAGTGCTTTTT
TCAGCGGTCTTGGTCTCGACTGGATCTCAACTCTCCCGAGGAGCAGGACGACAACGGAAACACCGGCGAG
CCATTTCGGTGCGTTCCTGGAGATGTGGCCAGAACCGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101813 representing NP_077118
Red=Cloning sites Green=Tags

MEDSEGTDGTEEDGCRAGGWFHVEAIITHGQRQVSSDEDEDETETGEDLDFIDNRVPGDGQEIPLQLYAQ
QTAQDDEATVQALKRKFVASPLSACSCIENDLSPRLDAISLNRKSEKAKRRLFETEPPDSGYGNTQMVVG
TPEEVTGDEESQGGRPVEDQEEERQGGDGEADLTVHTPQSGTDAAGSVLTLLRSSNLKATLLSKFKDLFG
VGFYELVRQFKSSKTACADWVVCAYGVYYAVAEGLKKLIQPHTQYAHIQVQTSSWGMVVFMLLRYNCAKN
RDSVSKNMSMLLNIPEKHMLIEPPKLRSTPAALYWYKTAMGNGSEVYGETPEWIVRQTLVGHSMEDEQFR
LSVMVQYAYDHDIVEESVLAFEYAQLADVDANAAAFLNSNCQAKYVKDAVTMCRHYKRAEREQMSMSQWI
TFRGNKVSEEGDWKPIVRFLRHQGVEFVSFLAAFKLFLKGVPKKNCIVFYGPADTGKSYFCMSLLQFLGG
AVISYANSSSHFWLQPLSDSKIGLLDDATPQCWSYIDIYLRNLLDGHPVSIDRKHKTLLQLKCPPLMITT
NTNPLEEDRWKYLRSRLTVFTFKNPFPFASPGEPLYPINNANWKCFFQRSWSRLDLNSPEEQDDNGNTGE
PFRCVPGDVARTV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001352
ORF Size 1929 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001352.1, NP_077118
RefSeq ORF 1929 bp
Locus ID 1489227
MW 72.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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