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env polyprotein (NC_001722) Virus Tagged ORF Clone
CAT＃: VC101749
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for env polyprotein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056844
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CNY 13,020.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101749 represents NCBI reference of NP_056844 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCAGGACGAAATCAATTGTTCGTTGTCATTCTGCTCACCTCTGCATGTCTGGTATATTGCTCAC AGTACGTGACCGTGTTTTATGGAATCCCGGCATGGAAAAATGCCTCCATTCCCCTCTTTTGTGCAACCAA AAATAGAGATACTTGGGGTACAATCCAATGCCTGCCCGATAACGACGACTACCAGGAGATAATACTGAAT GTGACCGAGGCATTCGACGCCTGGAACAACACAGTCACAGAACAGGCCGTCGAGGACGTATGGCACCTCT TCGAAACTTCCATAAAGCCTTGCGTGAAGCTGACACCTCTGTGTGTGGCTATGAACTGTTCCCGGGTGCA AGGCAATACCACAACGCCCAATCCACGCACTTCATCTTCTACAACCTCAAGACCTCCAACCTCAGCCGCC TCCATCATTAATGAGACAAGTAACTGCATCGAAAATAACACTTGCGCGGGGCTGGGGTATGAAGAGATGA TGCAGTGTGAGTTCAATATGAAAGGCCTTGAGCAGGACAAAAAGAGAAGATACAAGGACACCTGGTACCT TGAAGACGTCGTGTGCGATAACACCACTGCCGGAACATGTTACATGAGACACTGTAACACCTCCATTATC AAAGAGTCATGCGATAAGCACTATTGGGACGCCATGCGGTTTAGGTACTGTGCACCACCAGGGTTCGCAC TGCTGCGATGCAACGATACAAACTACTCAGGATTCGAACCTAAGTGTACTAAAGTCGTGGCTGCTTCCTG CACCAGGATGATGGAGACACAAACCTCCACATGGTTCGGCTTCAACGGCACTAGAGCTGAAAACAGGACA TATATCTATTGGCACGGGCGAGACAACAGAACAATTATCAGTTTGAATAAGTACTATAATCTGACGATGA GATGCAAGCGCCCTGGCAACAAGACTGTGCTCCCCATCACTTTGATGTCAGGCCTGGTATTCCATAGCCA GCCAATTAACACTAGACCCAGACAGGCCTGGTGCAGATTTGGTGGGAGGTGGCGAGAGGCGATGCAGGAG GTGAAACAGACCTTGGTCCAACATCCTCGGTACAAAGGGATAAATGATACAGGGAAAATCAATTTCACCA AGCCAGGCGCAGGTAGTGATCCGGAAGTGGCCTTCATGTGGACAAACTGCAGAGGCGAATTCCTGTATTG CAATATGACATGGTTTCTGAATTGGGTCGAAGACAAGAACCAGACCAGGAGAAATTACTGCCACATTAAG CAGATCATTAACACTTGGCATAAGGTTGGCAAGAACGTGTACCTTCCACCCCGCGAAGGAGAACTTGCGT GCGAGAGTACCGTGACCTCCATTATCGCCAACATCGATATCGATAAAAATCGGACACACACAAACATTAC CTTCTCTGCAGAGGTGGCGGAGCTTTACAGACTGGAACTGGGTGATTACAAGCTGATCGAAATTACACCA ATCGGATTTGCCCCTACAGACCAGAGGAGATACAGTAGCACTCCCGTCCGCAACAAGAGAGGTGTGTTTG TCCTGGGCTTCTTGGGATTCCTCGCTACAGCCGGCAGCGCTATGGGTGCTCGATCTCTGACTCTGTCTGC CCAGAGCAGGACCTTGCTTGCAGGAATCGTACAACAACAGCAGCAGCTTCTTGACGTAGTTAAGCGACAG CAGGAGATGCTGCGCCTCACTGTCTGGGGGACAAAAAATCTTCAGGCCAGAGTAACAGCCATTGAAAAAT ATCTGAAACACCAGGCACAGTTGAACAGTTGGGGCTGTGCCTTTAGACAGGTTTGCCACACAACGGTACC ATGGGTGAACGATTCCCTGAGCCCAGATTGGAAAAATATGACTTGGCAAGAGTGGGAGAAACAAGTGCGG TATTTGGAGGCTAATATCTCACAGTCTCTGGAAGAGGCACAGATCCAACAGGAGAAGAATATGTACGAGC TGCAGAAGCTGAATAGCTGGGACATCCTGGGAAACTGGTTTGATCTCACTAGTTGGGTCAAGTACATCCA GTATGGGGTGCACATCGTCGTCGGCATTATTGCCCTCCGGATCGCAATCTACGTAGTGCAACTTCTTAGC AGATTCCGAAAAGGCTACAGACCAGTTTTCAGTTCTCCTCCCGGATACCTGCAACAGATTCACATCCATA AGGACCGCGGCCAGCCAGCGAACGAAGGCACTGAGGAGGATGTAGGTGGAGACTCTGGCTATGATCTCTG GCCCTGGCCCATCAATTATGTGCAATTCCTGATTCATCTCCTGACTCGCCTGCTCATCGGCCTGTACAAC ATTTGTCGGGATCTGCTGTCCAAGAACAGTCCAACCCGCCGGTTGATCTCACAGAGCCTGACCGCTATCA GGGATTGGCTTAGGTTGAAGGCCGCACAGCTGCAGTACGGCTGCGAGTGGATACAGGAGGCTTTCCAAGC CTTCGCTAGGACCACCCGGGAGACTTTGGCTGGCGCATGGGGGTGGCTGTGGGAGGCGGCACGCAGAATT GGGAGGGGCATCCTCGCTGTGCCAAGGCGCATTCGCCAGGGCGCAGAATTGGCTTTGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101749 representing NP_056844
Red=Cloning sites Green=Tags MEPGRNQLFVVILLTSACLVYCSQYVTVFYGIPAWKNASIPLFCATKNRDTWGTIQCLPDNDDYQEIILN VTEAFDAWNNTVTEQAVEDVWHLFETSIKPCVKLTPLCVAMNCSRVQGNTTTPNPRTSSSTTSRPPTSAA SIINETSNCIENNTCAGLGYEEMMQCEFNMKGLEQDKKRRYKDTWYLEDVVCDNTTAGTCYMRHCNTSII KESCDKHYWDAMRFRYCAPPGFALLRCNDTNYSGFEPKCTKVVAASCTRMMETQTSTWFGFNGTRAENRT YIYWHGRDNRTIISLNKYYNLTMRCKRPGNKTVLPITLMSGLVFHSQPINTRPRQAWCRFGGRWREAMQE VKQTLVQHPRYKGINDTGKINFTKPGAGSDPEVAFMWTNCRGEFLYCNMTWFLNWVEDKNQTRRNYCHIK QIINTWHKVGKNVYLPPREGELACESTVTSIIANIDIDKNRTHTNITFSAEVAELYRLELGDYKLIEITP IGFAPTDQRRYSSTPVRNKRGVFVLGFLGFLATAGSAMGARSLTLSAQSRTLLAGIVQQQQQLLDVVKRQ QEMLRLTVWGTKNLQARVTAIEKYLKHQAQLNSWGCAFRQVCHTTVPWVNDSLSPDWKNMTWQEWEKQVR YLEANISQSLEEAQIQQEKNMYELQKLNSWDILGNWFDLTSWVKYIQYGVHIVVGIIALRIAIYVVQLLS RFRKGYRPVFSSPPGYLQQIHIHKDRGQPANEGTEEDVGGDSGYDLWPWPINYVQFLIHLLTRLLIGLYN ICRDLLSKNSPTRRLISQSLTAIRDWLRLKAAQLQYGCEWIQEAFQAFARTTRETLAGAWGWLWEAARRI GRGILAVPRRIRQGAELALL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001722 |
| ORF Size | 2580 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001722.1, NP_056844 |
| RefSeq ORF | 2580 bp |
| Locus ID | 1724717 |
| MW | 98.9 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101743 | Myc-DDK-tagged ORF clone of viral ORF for gag polyprotein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056837 |
CNY 6,370.00 |
|
| VC101744 | Myc-DDK-tagged ORF clone of viral ORF for vif protein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056839 |
CNY 3,800.00 |
|
| VC101745 | Myc-DDK-tagged ORF clone of viral ORF for vpx protein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056840 |
CNY 1,800.00 |
|
| VC101746 | Myc-DDK-tagged ORF clone of viral ORF for vpr protein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056841 |
CNY 1,320.00 |
|
| VC101747 | Myc-DDK-tagged ORF clone of viral ORF for tat protein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056842 |
CNY 3,800.00 |
|
| VC101748 | Myc-DDK-tagged ORF clone of viral ORF for rev protein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056843 |
CNY 3,800.00 |
|
| VC101750 | Myc-DDK-tagged ORF clone of viral ORF for nef protein [Human immunodeficiency virus 2], codon optimized for human cell expression, NP_056845 |
CNY 2,640.00 |
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