retropepsin (NP_705926) Virus Tagged ORF Clone
CAT#: VC101740
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for retropepsin [Human immunodeficiency virus 1], codon optimized for human cell expression, YP_001856241
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CNY 1,200.00
CNY 3,800.00
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC101740 represents NCBI reference of YP_001856241 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CCACAAGTCACGCTTTGGCAACGCCCTTTGGTGACAATTAAGATCGGGGGCCAGCTGAAGGAAGCACTCC TCGATACAGGGGCAGATGATACAGTGCTCGAGGAGATGAGCCTCCCTGGCAGATGGAAGCCCAAGATGAT AGGAGGGATTGGCGGCTTCATCAAAGTAAGGCAGTATGATCAGATCCTGATCGAGATATGTGGGCATAAG GCCATCGGAACTGTGTTGGTCGGCCCCACACCGGTGAACATTATTGGAAGAAATCTCCTCACTCAGATTG GGTGCACCTTGAATTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101740 representing YP_001856241
Red=Cloning sites Green=Tags PQVTLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHK AIGTVLVGPTPVNIIGRNLLTQIGCTLNF TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NP_705926 |
ORF Size | 297 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NP_705926.1, YP_001856241 |
RefSeq ORF | 297 bp |
MW | 10.8 kDa |
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