UL22 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100886

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein H [Human herpesvirus 2], codon optimized for human cell expression, NP_044491



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CNY 12,730.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100886 represents NCBI reference of NP_044491 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACCTGGTCTCTGGGTGGTGATGGGGGTGCTGGTGGGAGTGGCAGGAGGGCACGATACTTACTGGA
CTGAACAGATTGACCCATGGTTTCTCCACGGCCTCGGATTGGCTCGCACATACTGGCGCGATACCAACAC
CGGACGCCTCTGGCTGCCCAATACACCTGATGCGTCTGACCCTCAGCGGGGGCGACTTGCTCCACCCGGC
GAGCTGAATCTGACGACTGCTTCAGTCCCCATGCTTAGGTGGTATGCTGAAAGGTTCTGTTTCGTGCTCG
TCACCACAGCCGAATTTCCTCGAGATCCAGGCCAGCTTTTGTATATCCCTAAAACATATCTGCTGGGTAG
ACCACGCAATGCGAGCCTTCCGGAGCTCCCGGAGGCCGGCCCCACTAGCCGACCACCAGCCGAGGTTACA
CAGCTCAAGGGCCTGTCCCACAACCCAGGGGCCTCTGCTCTCCTGAGGTCAAGAGCATGGGTTACATTCG
CAGCCGCACCGGATCGGGAGGGGCTTACTTTTCCTCGCGGGGACGATGGGGCTACTGAGCGGCACCCAGA
CGGACGGAGGAATGCACCCCCCCCCGGTCCTCCCGCTGGAACCCCTAGACATCCTACGACTAATCTCAGC
ATCGCGCATCTCCACAATGCATCTGTCACATGGCTCGCCGCCAGAGGTCTTCTGAGAACCCCAGGGAGAT
ACGTTTATCTTTCCCCGAGTGCTTCAACATGGCCCGTTGGAGTGTGGACGACTGGAGGCCTGGCCTTTGG
TTGTGATGCTGCACTGGTGCGGGCCAGGTATGGAAAGGGCTTTATGGGCCTGGTGATCAGCATGAGAGAT
AGCCCTCCCGCTGAAATCATCGTAGTCCCCGCAGACAAAACCCTCGCCAGAGTCGGTAACCCAACAGATG
AGAACGCCCCCGCCGTGCTGCCAGGCCCCCCAGCTGGGCCGCGGTACAGGGTGTTTGTGTTGGGTGCCCC
TACCCCCGCTGATAATGGTAGCGCTCTCGATGCACTCCGACGGGTGGCAGGGTACCCCGAAGAATCCACC
AATTACGCCCAGTACATGTCAAGGGCTTACGCCGAGTTCCTCGGAGAGGACCCTGGGTCAGGAACGGACG
CCCGGCCTAGTCTGTTTTGGCGGTTGGCTGGGCTGCTCGCTAGCTCCGGCTTCGCGTTTGTGAATGCAGC
GCACGCACACGATGCCATACGCCTTTCTGATCTGTTGGGATTCCTGGCTCACAGCCGCGTCCTGGCCGGA
CTTGCTGCCCGAGGCGCTGCTGGGTGCGCTGCTGACTCCGTATTCCTCAACGTCAGCGTTCTGGACCCAG
CCGCAAGGCTCCGGCTTGAAGCACGACTGGGACACCTCGTCGCCGCAATCCTCGAGCGGGAGCAGAGCCT
CGTCGCTCATGCACTCGGTTACCAGCTTGCGTTTGTGCTGGATAGCCCTGCCGCATACGGCGCTGTAGCG
CCATCCGCAGCCAGACTTATCGACGCCCTGTACGCCGAATTTCTGGGTGGCCGCGCCCTGACTGCTCCCA
TGGTGCGCCGCGCACTCTTTTACGCGACCGCAGTTCTGAGAGCACCTTTTCTGGCTGGAGCACCCAGCGC
TGAACAGAGAGAGAGGGCCAGGCGAGGACTGCTGATCACCACTGCTCTGTGTACTTCTGATGTTGCAGCC
GCCACCCATGCTGATTTGAGAGCGGCACTTGCGAGGACCGACCACCAGAAGAACCTGTTCTGGCTTCCGG
ACCACTTCTCACCTTGCGCAGCCTCCCTTCGCTTCGATTTGGCCGAGGGTGGCTTCATTCTGGATGCCCT
GGCTATGGCGACCAGGTCCGACATACCAGCCGACGTGATGGCACAGCAGACAAGGGGGGTAGCTTCAGTG
TTGACAAGGTGGGCGCATTACAACGCTCTGATCCGCGCATTCGTACCCGAGGCTACCCATCAGTGTTCCG
GCCCTAGTCACAATGCCGAGCCCAGGATTCTCGTGCCTATTACGCATAATGCCTCTTATGTGGTGACACA
CACACCACTGCCCCGGGGCATCGGTTACAAACTGACTGGAGTCGACGTGAGAAGACCACTTTTCATTACA
TACCTTACAGCCACCTGCGAGGGGCACGCGCGCGAAATAGAACCCAAGAGGTTGGTGAGAACCGAAAACC
GACGGGACCTGGGACTGGTGGGGGCCGTCTTTCTTAGGTACACCCCGGCCGGAGAGGTGATGAGCGTGCT
CTTGGTTGACACTGACGCAACACAGCAGCAGCTGGCACAGGGACCAGTGGCGGGAACACCTAATGTGTTT
AGCTCCGACGTCCCGTCCGTGGCCCTGCTTTTGTTCCCTAACGGAACAGTAATCCACTTGCTCGCATTCG
ATACACTGCCTATAGCCACGATCGCCCCCGGGTTTCTGGCCGCCTCAGCACTCGGGGTAGTGATGATCAC
CGCGGCTCTCGCAGGCATTCTCCGCGTAGTGAGGACATGTGTCCCCTTTCTGTGGCGCCGAGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100886 representing NP_044491
Red=Cloning sites Green=Tags

MGPGLWVVMGVLVGVAGGHDTYWTEQIDPWFLHGLGLARTYWRDTNTGRLWLPNTPDASDPQRGRLAPPG
ELNLTTASVPMLRWYAERFCFVLVTTAEFPRDPGQLLYIPKTYLLGRPRNASLPELPEAGPTSRPPAEVT
QLKGLSHNPGASALLRSRAWVTFAAAPDREGLTFPRGDDGATERHPDGRRNAPPPGPPAGTPRHPTTNLS
IAHLHNASVTWLAARGLLRTPGRYVYLSPSASTWPVGVWTTGGLAFGCDAALVRARYGKGFMGLVISMRD
SPPAEIIVVPADKTLARVGNPTDENAPAVLPGPPAGPRYRVFVLGAPTPADNGSALDALRRVAGYPEEST
NYAQYMSRAYAEFLGEDPGSGTDARPSLFWRLAGLLASSGFAFVNAAHAHDAIRLSDLLGFLAHSRVLAG
LAARGAAGCAADSVFLNVSVLDPAARLRLEARLGHLVAAILEREQSLVAHALGYQLAFVLDSPAAYGAVA
PSAARLIDALYAEFLGGRALTAPMVRRALFYATAVLRAPFLAGAPSAEQRERARRGLLITTALCTSDVAA
ATHADLRAALARTDHQKNLFWLPDHFSPCAASLRFDLAEGGFILDALAMATRSDIPADVMAQQTRGVASV
LTRWAHYNALIRAFVPEATHQCSGPSHNAEPRILVPITHNASYVVTHTPLPRGIGYKLTGVDVRRPLFIT
YLTATCEGHAREIEPKRLVRTENRRDLGLVGAVFLRYTPAGEVMSVLLVDTDATQQQLAQGPVAGTPNVF
SSDVPSVALLLFPNGTVIHLLAFDTLPIATIAPGFLAASALGVVMITAALAGILRVVRTCVPFLWRRE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001798
ORF Size 2514 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001798.1, NP_044491
RefSeq ORF 2514 bp
Locus ID 1487306
MW 89.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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