VP1 (NC_012042) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100546 represents NCBI reference of YP_002586775 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCCTATCAAGAGACAGCCGGGTGGCTGGGTGTTGCCGGGGTATAAATACCTGGGACCTTTCAACC CCCTCGAAAATGGCGAACCGGTGAACAAGGCTGATAGAGCAGCCCAAGCCCATGATAAGTCTTACAGTGA AATAATAAAGTCAGGTAAGAATCCCTACCTGTATTTTAACAAGGCAGACGAGAAGTTCATTGATGACCTG AAAAACGATTGGTCACTGGGGGGCATCATTGGCAGTAGTTTCTTTAAACTGAAGAGAGCTGTCGCTCCAG CCCTGGGCAACAAAGAGAGGGCACAGAAGAGACATTTTTACTTTGCCAATTCCAACAAGGGGGCTAAGAA ACCAAAGAACAATGAGCCCAAACCAGGCACCTCTAAGATGAGTGAGAATGAAATCCAGGACCAACAACCC TCTGGTAGCATGGAAGAAAGAGGCGGCGGCGGCGGAGCAGTCGGCTCCGTAGGCGGGGGCAAGGGTTCAA GTGTCGGCATCAGCACTGGAGGGTGGGTCGGAGGGTCTTATTTTACTGACTCCTACGTGATCACCAAGAA CACACGGCAATTCTTGGTCAAGATTCAGAACGATCATAAGTATAGAACAGAGAATATCATCCCTAGCAAC GCAGGAGGGAAGTCACAGCGATGTGTGAGCACCCCATGGAGTTACTTCAATTTTAACCAGTACAGTTCCC ACTTCAGTCCCCAGGATTGGCAGCATTTGACTAACGAGTATAAACGCTTTAAACCAAGAAAAATGCATGT GAAAATTTACAATCTGCAGATTAAACAGATTCTCAGCAATGGAGCCGATACAACATATAATAATGATCTT ACCGCAGGAGTACATATTTTCTGTGATGGCGAGCACGCTTACCCAAACGCAACTCACCCCTGGGACGAGG ACGTGATGCCAGAACTTCCCTACGAAACCTGGTACCTGTTTCAGTATGGCTACATTCCAGTTATCCACGA ACTGGCTGAAATGGAGGACGCGAACGCTGTTGAAAAGGCAATCGCACTGCAGATACCCTTCTTTATGCTG GAGAATTCCGACCATGAAGTGCTTCGGACAGGCGAGAGTACGGAATTCACGTTCGATTTTGATTGCGAAT GGATCAACAACGAGCGCGCCTATATTCCCCCTGGCCTGATGTTCAACCCAAAGGTTCCCACTAGGAGAGC GCAATACATCAGGCAGCACGGGAACACGGCCTCCTCAAACACAAGAATTCAACCATATGCGAAGCCAACT TCCTGGATGACTGGACCTGGCCTGCTGAGTGCCCAGAGGGTTGGACCAGCCGGCTCTGATACCGCCAGCT GGATGGTGGTGGTCAACCCAGACGGAGCCGCTGTTAATTCCGGGATGGCCGGCGTTGGGTCCGGTTTCGA CCCCCCAAGCGGATCCCTGCGCCCCACGGATCTGGAGTATAAGATTCAGTGGTATCAGACCCCAGCCGGG ACAAATAGCGACGGCAATATTATCAGCAACCCGCCTCTGTCTATGCTTCGGGACCAGGCCCTGTACCGGG GAAATCAGACCACCTACAACCTGTGTAGCGACGTATGGATGTTTCCCAATCAGATTTGGGACAGATACCC CATAACACGGGAGAACCCAATCTGGTGTAAGAAACCTCGCAGTGATAAAAACACCATCATAGACCCGTTC GACGGGACACTGGCCATGGATCACCCGCCGGGCACCATTTTTATTAAGATGGCCAAGATTCCGGTCCCTT CTAACAACAATGCTGATAGTTACCTGAACATCTACTGTACCGGCCAAGTTAGTTGTGAGATCGTGTGGGA AGTGGAACGGTACGCTACTAAAAACTGGCGCCCTGAACGGAGGCATACCGCACTCGGTTTGGGGATCGGC GGAGAGGAGAACGTTAATCCTACCTACCATGTGGACAAGAATGGAAAATACATTCAGCCCACTACTTGGG ACATGTGCTACCCCATTAAGACTAATATTAATAAAGTGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100546 representing YP_002586775 Red=Cloning sites Green=Tags MPPIKRQPGGWVLPGYKYLGPFNPLENGEPVNKADRAAQAHDKSYSEIIKSGKNPYLYFNKADEKFIDDL KNDWSLGGIIGSSFFKLKRAVAPALGNKERAQKRHFYFANSNKGAKKPKNNEPKPGTSKMSENEIQDQQP SGSMEERGGGGGAVGSVGGGKGSSVGISTGGWVGGSYFTDSYVITKNTRQFLVKIQNDHKYRTENIIPSN AGGKSQRCVSTPWSYFNFNQYSSHFSPQDWQHLTNEYKRFKPRKMHVKIYNLQIKQILSNGADTTYNNDL TAGVHIFCDGEHAYPNATHPWDEDVMPELPYETWYLFQYGYIPVIHELAEMEDANAVEKAIALQIPFFML ENSDHEVLRTGESTEFTFDFDCEWINNERAYIPPGLMFNPKVPTRRAQYIRQHGNTASSNTRIQPYAKPT SWMTGPGLLSAQRVGPAGSDTASWMVVVNPDGAAVNSGMAGVGSGFDPPSGSLRPTDLEYKIQWYQTPAG TNSDGNIISNPPLSMLRDQALYRGNQTTYNLCSDVWMFPNQIWDRYPITRENPIWCKKPRSDKNTIIDPF DGTLAMDHPPGTIFIKMAKIPVPSNNNADSYLNIYCTGQVSCEIVWEVERYATKNWRPERRHTALGLGIG GEENVNPTYHVDKNGKYIQPTTWDMCYPIKTNINKVL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_012042
ORF Size	2001 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_012042.1, YP_002586775
RefSeq ORF	2001 bp
Locus ID	7755624
MW	74.9 kDa

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