L2 (NC_003266) Virus Tagged ORF Clone

CAT＃: VC100473

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for core protein V [Human adenovirus E], codon optimized for human cell expression, YP_068029

Datasheet

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CNY 4,090.00

货期*

5周

规格

Product images

经常一起买 (4)

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100473 represents NCBI reference of YP_068029 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAAAAGAAAGTTCAAGGAGGAAATGCTTCAGGTTATCGCACCAGAAATTTACGGGCCAGCCGCGG CGGTAAAGGACGAGAGGAATCCCAGAAAGATCAAGCGCGTGAAGAAGGACAAAAAGGAGGAGGATGATGT CGATGATATGGTTGAGTTTGTGAGAGAATTCGCACCTCGCAGACGAGTGCAGTGGCGAGGTAGGAAGGTG AGGCCCGTGCTTAGACCTGGGACTACAGTCGTGTTTGCCCCAGGTGAACGCTCCGGAACAACCTCTAAGC GGAGTTACGACGAGGTGTATGGAGATGAAGATATCCTGGAGCAAGCTGCCGAAAGACTCGGAGAATTTGC ATACGGGAAGCGGAACAGACTCGCACCTCTGAAAGAAGAAGTAGTCAGCATTCCCCTCGACCACGGGAAC CCTACCCCGAGTCTCAAACCTGTCACACTCCAGCAAGTACTTCCATCCGCTGCACCTAGACGAGGATTGA AGCGGGAAGGCGAAGACGTGTATCCGACTATGCAACTGATGGTTCCCAAGAGGCAGAAACTTGAGGACGT GTTGGAGACTATGAAGGTGGACCCAGACGTTCAACCGGAGGTGAAAGTGCGGCCCATCAAGCAAGTGGCC CCCGGCCTTGGCGTGCAAACCGTTGATATAAAGATACCAACCGAGCCGATGGAAACTCAGACAGAGGTGG TCAAGCCCATTACCAGTACAATGGAAGTCCAGACTGACCCCTGGATGCCTGCCGCCCCACGGAAACCCCG ACGCAAGTATGGTGCTGCCAGTCTGCTGATGCCGAACTACGCCCTTCACCCTTCAATTATTCCGACACCC GGATATAGGGGGACGCGATTCTACCATGGCTATACCGGTTCAAGAAGAAGGAAGACGACTACTAGGAGAA GACGACGCCGCACTGCCGCAACACCCGCCGCCGCCTTGGTGCGAAGAGTGTATCGGAGGGGGAGAGCCCC TCTTACCCTGCCTAGAGCTCGGTACCATCCCAGCATCACTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100473 representing YP_068029 Red=Cloning sites Green=Tags MSKRKFKEEMLQVIAPEIYGPAAAVKDERNPRKIKRVKKDKKEEDDVDDMVEFVREFAPRRRVQWRGRKV RPVLRPGTTVVFAPGERSGTTSKRSYDEVYGDEDILEQAAERLGEFAYGKRNRLAPLKEEVVSIPLDHGN PTPSLKPVTLQQVLPSAAPRRGLKREGEDVYPTMQLMVPKRQKLEDVLETMKVDPDVQPEVKVRPIKQVA PGLGVQTVDIKIPTEPMETQTEVVKPITSTMEVQTDPWMPAAPRKPRRKYGAASLLMPNYALHPSIIPTP GYRGTRFYHGYTGSRRRKTTTRRRRRRTAATPAAALVRRVYRRGRAPLTLPRARYHPSITI TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_003266
ORF Size	1023 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_003266.2, YP_068029
RefSeq ORF	1023 bp
Locus ID	2958475
MW	39.0 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100462	Myc-DDK-tagged ORF clone of viral ORF for control protein E1A [Human adenovirus E], codon optimized for human cell expression, YP_068018	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100463	Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus E], codon optimized for human cell expression, YP_068019	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100464	Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 55K [Human adenovirus E], codon optimized for human cell expression, YP_068020	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
VC100465	Myc-DDK-tagged ORF clone of viral ORF for capsid protein IX [Human adenovirus E], codon optimized for human cell expression, YP_068021	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100466	Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus E], codon optimized for human cell expression, YP_068022	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,510.00
VC100467	Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus E], codon optimized for human cell expression, YP_068023	10 ug 产品选项验证失败所有选项都是必需的	CNY 18,050.00
VC100468	Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus E], codon optimized for human cell expression, YP_068024	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
VC100469	Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 52K [Human adenovirus E], codon optimized for human cell expression, YP_068025	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,660.00
VC100470	Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pIIIa [Human adenovirus E], codon optimized for human cell expression, YP_068026	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,130.00
VC100471	Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus E], codon optimized for human cell expression, YP_068027	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
VC100472	Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pVII [Human adenovirus E], codon optimized for human cell expression, YP_068028	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100474	Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pX [Human adenovirus E], codon optimized for human cell expression, YP_068030	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100475	Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus E], codon optimized for human cell expression, YP_068031	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100476	Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus E], codon optimized for human cell expression, YP_068032	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,060.00
VC100477	Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus E], codon optimized for human cell expression, YP_068033	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100478	Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus E], codon optimized for human cell expression, YP_068034	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,180.00
VC100479	Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus E], codon optimized for human cell expression, YP_068035	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,070.00
VC100480	Myc-DDK-tagged ORF clone of viral ORF for protein 33K [Human adenovirus E], codon optimized for human cell expression, YP_068036	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100481	Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 22K [Human adenovirus E], codon optimized for human cell expression, YP_068037	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100482	Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVIII [Human adenovirus E], codon optimized for human cell expression, YP_068038	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100483	Myc-DDK-tagged ORF clone of viral ORF for control protein E3 125K [Human adenovirus E], codon optimized for human cell expression, YP_068039	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100484	Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-alpha [Human adenovirus E], codon optimized for human cell expression, YP_068040	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100485	Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 gp19K [Human adenovirus E], codon optimized for human cell expression, YP_068041	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100486	Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-beta [Human adenovirus E], codon optimized for human cell expression, YP_068042	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100487	Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-delta [Human adenovirus E], codon optimized for human cell expression, YP_068043	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100488	Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-alpha [Human adenovirus E], codon optimized for human cell expression, YP_068044	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100489	Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-beta [Human adenovirus E], codon optimized for human cell expression, YP_068045	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100490	Myc-DDK-tagged ORF clone of viral ORF for control protein E3 147K [Human adenovirus E], codon optimized for human cell expression, YP_068046	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100491	Myc-DDK-tagged ORF clone of viral ORF for protein U, partial [Human adenovirus E], codon optimized for human cell expression, YP_068047	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100492	Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus E], codon optimized for human cell expression, YP_068048	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,230.00
VC100493	Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf6/7 [Human adenovirus E], codon optimized for human cell expression, YP_068049	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100494	Myc-DDK-tagged ORF clone of viral ORF for control protein E4 34K [Human adenovirus E], codon optimized for human cell expression, YP_068050	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100495	Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf4 [Human adenovirus E], codon optimized for human cell expression, YP_068051	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100496	Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf3 [Human adenovirus E], codon optimized for human cell expression, YP_068052	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100497	Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf2 [Human adenovirus E], codon optimized for human cell expression, YP_068053	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100498	Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf1 [Human adenovirus E], codon optimized for human cell expression, YP_068054	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100499	Myc-DDK-tagged ORF clone of viral ORF for protein 136K [Human adenovirus E], codon optimized for human cell expression, YP_001661328	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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