GARNL1 (RALGAPA1) (NM_001283044) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1; TULIP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001283044, the custom clone sequence may differ by one or more nucleotides ATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTAGACACCAAGAAGGACG CACTGACTCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGATCTTAAACAGTTTTT CGACCAACATTTTTCACATATATACTATGTGTTCTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTGGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC TTCTTCCAGAAAGAATTCATCAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCTACTTCA CACAGGAAACTCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTTTCTTACTATGGTTGCAAGCTCTTCAG AATAACTGTAGCAAAGAACAGCTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCACCACAGTCTG AACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTTCAAGAAACTCAAGTCACTAT AGAAGAAATCACTCCTCTTGTCCCCCCACAATCAGGAGATAAAGGGCAAGAAGATCTCACAAGCTATTTT CTTGAAGCACTTCTAAAATACATAGTCATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAATATTACTTGCCTTATATTTTTCCAAACATCTG TAAGGAAAACAGTTTATATCATCCTATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTG ATAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTCATTAAGGCTCGTGTTA TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATACAGGACCTCATATTCCTGGGAT GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTAGTATCCAGAGAAGAAAGCAAA AATGATAATGCTGATAAAACAGACAGAACTACAGAACCCGAACAGTCTCATTCCAATACAAGCACTCTCA CGGAGCGAGAACCTAGCTCATCTAGTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGT TCGCAGAGTTTTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTATATCAAGAATGGATCCAACAAGAGG AAAAACCTTTGTTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCAGACCTCCCTTGCATTGAAAA TGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAAAGAGAAGAGGAAAATGGGACCAATACTGCT GATCATGTTCGAAATTCCAGTTGGGCAAAAAACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAG AAGCCACAGAACAAAACATACGAGCTGGTACCCAGGCAGTTTTGCAGGTGTTTATTATAAACTCATCAAA TATATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAACACACAGATATGTGTAAACGC ATTCTTAACATTTATCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAACAGATGCTGC TTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCACAAGCTTTTCTACAGTTCCAAGGGAA AAAAAATATGACCTTGGCAGGTCGACTTGCAGGACCACTTTTCCAGACCCTTATAGTTGCCTGGATCAAA GCAAACCTAAATGTGTACATCTCCCGAGAACTTTGGGATGACTTACTGTCAGTATTGTCATCGCTGACCT ATTGGGAAGAGTTGGCCACTGAGTGGTCACTGACTATGGAGACATTAACTAAAGTTTTAGCTAGGAATTT ATATAGTTTGGATCTCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAGCACAAAGGG AAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGTGATC AGCCTGGCCAAGCCCCAATGAGACAGAGGAGTGCAACAACCACTGGTTCTCCAGGAACCGAAAAGGCGAG GAGTATAGTACGGCAAAAAACTGTCGCCATGAGAAGCCGATCCATTGGTGAATGTGCTCTGCCATCGGCC TATATACGCAGTGCTAAAAGTGCTCCTGTTCTGATCCATACTTCCAAACCCTTCTTGCCTGATATTGTTC TCACTCCCCTTTCTGATGAGCTTTCAGATATTGATGATGCTCAAATACTTCCCCGCTCAACTAGAGTCAG ACATTTTTCACAAAGTGAAGAAACTGGAAATGAAGTTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCT CGAAGTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAACGAGCCAAAGTCAATAAAGAGGACA TGAGCCAAAAACTGCCTCCTCTTAATAGTGATATTGGCGGCAGCAGTGCTAATGTTCCTGATCTGATGGA TGAGTTTATAGCAGAACGACTTCGAAGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGTAGTCCAGGC AGCCTGGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGCCCTATTGATGACC CAGGTGTGCCCTCAGAATGGACTTCTCCTGCCAGTGCAGGGAGCAGTGATCTTATCAGCTCAGATAGTCA TTCGGATTCTTTCAGCGCTTTCCAATATGATGGCCGAAAATTTGACAATTTTGGCTTTGGAACCGACACT GGGGTTACGTCCTCTGCTGATGTGGATTCAGGTTCTGGCCATCATCAGAGTGCTGAAGAGCAGGAAGTGG CTAGTCTAACTACTCTTCATATAGATTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGT TGCAACTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGCTCCAGGTCACAGACTCCT TCCCCTTCTACATTGAATATAGATCACATGGAACAGAAGGATCTGCAGCTCGACGAGAAGCTCCACCACT CTGTTCTTCAGACACCAGATGATCTAGAAATTAGTGAATTTCCATCAGAATGTTGTAGTGTGATGGCAGG AGGTACTCTGACTGGATGGCATGCTGATGTTGCTACTGTAATGTGGCGAAGAATGCTAGGCATTTTGGGA GATGTAAATTCAATCATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGA ATCTAGCTAAGATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTCCCCTTCTCCACCAGTTTT AATTCCTCCACTGAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAATATAAACAA GGTAAATTACATGCATATAAACTTATTTGTAATACAATGAAAAGAAGACAAGATGTTTCTCCAAATAGAG ATTTTCTAACACATTTCTACAATATAATGCATTGTGGATTACTTCATATTGACCAGGATATTGTCAATAC AATCATCAAACACTGCTCACCTCAATTTTTTTCACTTGGTTTGCCTGGTGCCACAATGCTTATTATGGAT TTTATTGTAGCAGCTGGTAGAGTGGCTTCTTCAGCTTTTCTCAATGCACCAAGAGTAGAAGCACAAGTTC TTCTGGGATCTTTGGTTTGCTTTCCCAACTTATATTGTGAACTGCCTTCTCTTCATCCCAACATTCCTGA TGTTGCTGTGTCTCAGTTTACAGATGTTAAGGAACTTATAATCAAAACTGTATTAAGCTCGGCAAGAGAT GAGCCCTCTGGTCCTGCACGATGTGTAGCACTTTGTAGTTTAGGTATTTGGATTTGTGAAGAACTAGTCC ATGAGTCTCATCATCCTCAAATTAAGGAAGCTCTGAATGTGATTTGTGTTTCCTTAAAGTTTACTAATAA AACAGTAGCCCACGTAGCTTGTAACATGCTTCACATGCTGGTTCATTATGTACCTAGACTTCAGATTTAC CAGCCTGATTCTCCCTTGAAAATTATTCAAATCCTAATAGCTACCATCACCCATCTTTTACCAAGTACAG AGGCTTCATCTTATGAAATGGACAAGAGGTTGGTAGTATCTTTACTTCTCTGCCTTCTGGACTGGATCAT GGCCTTACCTCTAAAGACACTGCTCCAACCATTTCATGCTACGGGAGCAGAAAGCGATAAAACAGAAAAA TCTGTTCTCAATTGCATTTATAAGGTTTTACATGGGTGTGTTTATGGAGCTCAGTGTTTTAGCAATCCAA GGTATTTTCCCATGAGCCTCTCTGATTTGGCATCTGTAGATTATGATCCTTTTATGCATTTGGAAAGTCT GAAAGAGCCTGAGCCTCTGCACTCTCCTGACTCAGAACGATCTTCTAAACTCCAGCCAGTAACAGAAGTG AAAACTCAAATGCAGCATGGATTAATCTCTATAGCAGCCCGCACTGTTATTACACATCTGGTAAATCACC TGGGCCATTATCCAATGAGCGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCACGACAATCA TTACAGTGAAAGTACTGAACTTTCTCCTGAACTCTTTGAGAGTCCAAATATCCAGTTCTTTGTGTTAAAT AATACAACCTTAGTGTCCTGTATCCAGATCAGATCAGAAGAGAATATGCCTGGAGGAGGTTTATCTGCTG GCCTTGCATCAGCCAATTCAAATGTCAGAATCATAGTACGTGATCTCTCTGGAAAATATTCATGGGATTC TGCTATACTGTATGGCCCACCTCCTGTAAGTGGCTTGTCAGAACCTACATCTTTCATGCTTTCATTGTCT CACCAAGAGAAGCCAGAAGAGCCTCCGACATCTAATGAATGCTTAGAAGATATAACCGTAAAAGATGGAC TTTCTCTCCAGTTTAAAAGATTTAGAGAAACTGTACCAACTTGGGATACAATAAGAGATGAAGAAGATGT TCTTGATGAGCTCTTGCAGTATTTGGGTGTTACTAGTCCTGAATGCTTACAGAGAACTGGAATCTCACTT AATATTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAAGAAAATGATGTTATTAATGCTATCCTTA AGCAACATACAGAAGAAAAAGAATTTGTTGAGAAGCACTTTAATGACTTAAACATGAAAGCTGTGGAACA AGATGAACCAATACCTCAAAAACCTCAGTCAGCATTTTATTATTGCAGATTGCTTCTTAGTATATTGGGA ATGAATTCCTGGGACAAACGGAGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTA GGAACTTGGATTCAAGGCAGTGCCGAGAGACACACAAGATTGCAGTATTTTATGTTGCTGAAGGACAAGA AGACAAACACTCCATTCTCACCAATACAGGAGGAAGTCAAGCATATGAAGATTTTGTAGCTGGTCTTGGT TGGGAGGTAAATCTTACAAACCATTGTGGTTTTATGGGAGGACTACAAAAAAACAAAAGCACTGGATTGA CCACTCCATATTTTGCTACCTCTACAGTAGAGGTAATATTTCACGTGTCAACAAGAATGCCTTCTGATTC TGATGATTCTTTGACCAAAAAATTGAGACATTTGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCAT ACTAGAGACTACAGGAGAGGAATTATTCCCACAGAATTTGGTGATGTCCTTATTGTAATATATCCAATGA AAAATCACATGTTCAGTATTCAGATAATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGG TGCTATTGTGAATGGAAAGGTTCTACCCATTATGGTTAGAGCAACAGCTATAAATGCAAGCCGTGCTCTG AAATCTCTGATTCCATTGTATCAAAACTTCTATGAGGAGAGAGCACGATACCTGCAAACAATTGTCCAGC ACCACTTAGAACCAACAACATTTGAAGATTTTGCAGCACAGGTTTTTTCTCCAGCTCCCTACCACCATTT ACCATCTGATGCCGATCATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001283044
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001283044.1, NP_001269973.1
RefSeq Size	6666 bp
RefSeq ORF	6252 bp
Locus ID	253959
UniProt ID	Q6GYQ0
Gene Summary	This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (4) contains an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Variants 4 and 7 encode the same isoform (4). Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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