Dicer (DICER1) (NM_001291628) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291628, the custom clone sequence may differ by one or more nucleotides ATGAAAAGCCCTGCTTTGCAACCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAA TGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATA TCAGGTTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGGAAG ACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCAGAAATGGAA AAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCTGTCAGAACTCATTC AGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAACCAA GAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACT TATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCG AGAAATTATGAAGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCTTCCATTTTA AATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTTAAGAGTAATG CTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCATGTGAGATTGTGGTGGATTG TGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGCTGATGGAATTAGAAGAAGCACTTAATTTT ATCAATGATTGTAATATATCTGTACATTCAAAAGAAAGAGATTCTACTTTAATTTCGAAACAGATACTAT CAGACTGTCGTGCCGTATTGGTAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGAATGATGGTAAG AGAACTACAGAAATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTATTGTTTACAGACACT TTCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTTGACCTGAAATTTGTAA CTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATGAGCGACAGCAGTTTGAAAG CGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGTCATGGAGTGATTCTGAGGATGATGATGAG GATGAAGAAATTGAAGAAAAAGAGAAGCCAGAGACAAATTTTCCTTCTCCTTTTACCAACATTTTGTGCG GAATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCTTAAACAGATTGATAAAGGAAGCTGGCAAACA AGATCCAGAGCTGGCTTATATCAGTAGCAATTTCATAACTGGACATGGCATTGGGAAGAATCAGCCTCGC AACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGGAAATTTCGAGCACATGAGACCA ACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCAAAATGCAACTTGGTGGTTCGTTT TGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAGGAAGAGCAAGGGCACCCATCTCTAATTAT ATAATGTTAGCGGATACAGACAAAATAAAAAGTTTTGAAGAAGACCTTAAAACCTACAAAGCTATTGAAA AGATCTTGAGAAACAAGTGTTCCAAGTCGGTTGATACTGGTGAGACTGACATTGATCCTGTCATGGATGA TGATGACGTTTTCCCACCATATGTGTTGAGGCCTGACGATGGTGGTCCACGAGTCACAATCAACACGGCC ATTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGATCCGTTTACTCATCTAGCTCCTAAATGCA GAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTTTATCTGCCAATTAACTCACCTCTTCGAGC CTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCTGAAAGAGTTGTAGCTCTCATTTGCTGTGAG AAACTGCACAAAATTGGCGAACTGGATGACCATTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAG AGGAGCTTGATTTGCATGATGAAGAAGAGACCAGTGTTCCAGGAAGACCAGGTTCCACGAAACGAAGGCA GTGCTACCCAAAAGCAATTCCAGAGTGTTTGAGGGATAGTTATCCCAGACCTGATCAGCCCTGTTACCTG TATGTGATAGGAATGGTTTTAACTACACCTTTACCTGATGAACTCAACTTTAGAAGGCGGAAGCTCTATC CTCCTGAAGATACCACAAGATGCTTTGGAATACTGACGGCCAAACCCATACCTCAGATTCCACACTTTCC TGTGTACACACGCTCTGGAGAGGTTACCATATCCATTGAGTTGAAGAAGTCTGGTTTCATGTTGTCTCTA CAAATGCTTGAGTTGATTACAAGACTTCACCAGTATATATTCTCACATATTCTTCGGCTTGAAAAACCTG CACTAGAATTTAAACCTACAGACGCTGATTCAGCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTC CAGCACTTTGGATATTGACTTTAAATTCATGGAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCC AGTACAAAGTATACAAAAGAAACACCCTTTGTTTTTAAATTAGAAGATTACCAAGATGCCGTTATCATTC CAAGATATCGCAATTTTGATCAGCCTCATCGATTTTATGTAGCTGATGTGTACACTGATCTTACCCCACT CAGTAAATTTCCTTCCCCTGAGTATGAAACTTTTGCAGAATATTATAAAACAAAGTACAACCTTGACCTA ACCAATCTCAACCAGCCACTGCTGGATGTGGACCACACATCTTCAAGACTTAATCTTTTGACACCTCGAC ATTTGAATCAGAAGGGGAAAGCGCTTCCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCT GCAGAATAAACAGATACTGGTTCCAGAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAA GCTGTTTGTCTCCCCAGCATACTTTATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAAGAGCCCAGA CTGCCAGCGATGCTGGCGTGGGAGTCAGATCACTTCCTGCGGATTTTAGATACCCTAACTTAGACTTCGG GTGGAAAAAATCTATTGACAGCAAATCTTTCATCTCAATTTCTAACTCCTCTTCAGCTGAAAATGATAAT TACTGTAAGCACAGCACAATTGTCCCTGAAAATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAG AAAATCATGACCAAATGTCTGTGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGA AGTTTCAGCAGATCTTACAGCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGAT TTAGCTAACAGAGACTTTTGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCGTGCAACCAA CTACCTCATATTCCATTCAGAATTTATACAGTTACGAGAACCAGCCCCAGCCCAGCGATGAATGTACTCT CCTGAGTAATAAATACCTTGATGGAAATGCTAACAAATCTACCTCAGATGGAAGTCCTGTGATGGCCGTA ATGCCTGGTACGACAGACACTATTCAAGTGCTCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTG GGTACTCCTCAAGGACTCTTGGCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAG TGATGGATTTAACCTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATAT CTATTTTGCACTTACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACT GTAATCTGTATCGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGGTGTCAATATTTGATCCCCC TGTGAATTGGCTTCCTCCTGGTTATGTAGTAAATCAAGACAAAAGCAACACAGATAAATGGGAAAAAGAT GAAATGACAAAAGACTGCATGCTGGCGAATGGCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGG AGGAGAGCCTGATGTGGAGGGCTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCA GGAACATATCAGATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCT CCTTTTTCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTAGTATGCCAT TTTCATCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCTATCTGGATCCTAGCAAAGC TGTTGAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCATCAGAAGAAAACTGTGGTGTTGACACGGGA AAGCAGTCCATTTCTTACGACTTGCACACTGAGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGG AAGCCCTGCTGGGCTGCTATTTAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGG GCTGAAGGTGCTCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTC AACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCTCTTCTGTAT TGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTGATCATCCAGATGCAGATAA AACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAAAGAAAATCAACTACAGATTCAAGAATAAG GCTTACCTTCTCCAGGCTTTTACACATGCCTCCTACCACTACAATACTATCACTGATTGTTACCAGCGCT TAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGGCAGCA CTCCCCGGGGGTCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTGGCTGTA AAGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATGACTTTGTGC AGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGAGATCTGAGGAGGATGAAGA GAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATATTTTTGAGTCGCTTGCTGGTGCCATTTAC ATGGATAGTGGGATGTCACTGGAGACAGTCTGGCAGGTGTACTATCCCATGATGCGGCCACTAATAGAAA AGTTTTCTGCAAATGTACCCCGTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAAACTGCCAAATT TAGCCCGGCTGAGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGAAAGGGGAAATTT AAAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTCCGAAGCCTCAAAG CTAATCAACCTCAGGTTCCCAATAGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001291628
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291628.1, NP_001278557.1
RefSeq Size	10206 bp
RefSeq ORF	5769 bp
Locus ID	23405
UniProt ID	Q9UPY3
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (5) lacks an internal segment in the 5' UTR, compared to variant 2. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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