TJP1 (NM_001301026) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZO-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301026, the custom clone sequence may differ by one or more nucleotides ATGGAGAGAAATACGTTTTCAATGGATTGCCACAGTAAGAGCACAGCAATGGAGGAAACAGCTATATGGG AACAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGA TAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAA GGACAGCTACAGGAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTT TTGCTGTTCAGCAACTAAGGAAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCA AATACCAGTAAGTCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATA CATGATCCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAA GTGCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAA ACCTACTAAAGTCACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATA TTTGTTAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTAT TGAAGATAAATGGTACTGTGACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAA AGGCAAATTAAAAATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGAC AGCATCCACTCTGCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATT CTGGTCGATCACACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCTTC TGATCATTCCAGGCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGAATT TCTAAACCTGGGGCTGTCTCAACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAG TTACAGTTGAAAGAAATGAGAAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGG GCAACCAGATGTGGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGG ATTCTTCGGCCCAGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTG GAAATGATGTTGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGCTTAGAGGA AGGTGATCAAATTCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCCGTCCTTTTC CTGCTTGACCTCCCTAAAGGAGAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCA TTGTAGAATCAGATGTAGGAGATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTA TGGACTTAGTTTTAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCT TGGCTTGCTATTCGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAG CTGAGCAGCTAGCCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGACTTCTGGAG ATTCAGAGGTCTTCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTGTCCGCTCAGCCTGTT CAAACAAAGTTTCCAGCTTATGAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTT TTGGACCAATAGCTGATGTTGCCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAA GAGTGAACCACGAGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAA ATCATAGATCAAGACAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCC AGTGGTATCCAATTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGAATGAGGTT ATGTCCAGAATCTCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCATAAACTTCGTAAAAATAATCAC CATCTTTTTACAACTACAATTAACTTAAATTCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAA TTCAACAACAGCAAAACCAGCTGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCT TGATTTGCATGATGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGAC AGTAGACACACTTCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATG AAACTCTTAATGATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGA GGACTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCTTACTCACCACAAGCGCAGCCACAACCA ATTCATAGAATAGACTCCCCTGGATTTAAGCCAGCCTCTCAACAGGTGTATAGAAAGGATCCATATCCCG AGGAAATGATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCCAGGGCACAGGCCAGACAA AGAGCCTAATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGACCTCGAGCAG CCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACATCGTCTGCGAT ACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAAACAGCCCTACCCATCTCG GCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCCGAAGAGTCCTCAGAACGA GGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGACCACGTTACGAACAGGCAC CTAGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACACACATGGTAGACTCAGACC GGAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAGCAATATTCA CGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCTCTCCATGGTG CTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCTGCCTTCAAACACCAAACC ACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAGCCACAGTCTGTACTCACC AGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGGATGTAAATGACACTGGCA GTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGGTCCCAAACCCACTTCTCA GAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAAAAACCTCAACTGAAGCCA CCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGAAAACAGCTGT CATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGCCAGCCATCTCTCCGAGCC TGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCAAAGGGAAAGCCTCCTGAA GCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGGCCACTCCCCCTCCTCCTC CATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCTCCACATACATTCTAAGGG AGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAACCAGACCCACCT CCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAGGCAGCTTTCTATC CCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCAGAAAACAGTCACTCCAGC ATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAACGCAAGTTTGAAAGTCCT AAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGTCTTCAAAAACTCCCACTT CTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGACAGTGGAGTTGAAACTTT CTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTGCCTAAAGCTATTCCTGTG AGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGGCCACAGCCCGAGGCATAT TTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAATTATCCCTCAAGGAGCCAT TCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGCATCCTTCCACCTTTAGAT AAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCCCATGGCCTCAAGTTCCTGAAGC CTGTGGAGCTGCGCTTACCACACTGTGCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCTAAAATCATC CGACTCCTCGTCGGGTGATCCTAAAACCTGGCAAAACAAGTGTCTTCCCGGAGATCCAAATTATCTCGTT GGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301026
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301026.1, NP_001287955.1
RefSeq Size	7570 bp
RefSeq ORF	5079 bp
Locus ID	7082
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection
Gene Summary	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (4) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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