EIF4G1 (NM_001291157) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291157, the custom clone sequence may differ by one or more nucleotides ATGAACACGCCTTCTCAGCCCCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCT CCCGAGTGCAGAGTGCAGCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACCCTGCTGGATCCCAAGT AATGATGATCCCTTCCCAGATCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGT TCCACATACGTTGTCCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAA GCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTTCCCACTGG CGTGGCCCCCGCCCCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATC CGAATTCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCT CCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGC TGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCA GAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAAC CGGGGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGT AGAAGAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTC GCCGAACCCATACTGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTC CTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATC TGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCC CCTGTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGG ACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAATGGCGCCCCCCACCAT CCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAA GAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGC TCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAAGT GGCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTT CTGGATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATC CAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAA GGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAG TGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCT TTGCCAGTATGCAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAAC ACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACTCCATCCTTT GCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCC GTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGATCATTGC CACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGG ACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGG TGCGCTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGC CATCGACACCGAGGAACGCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAAC TTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAA CAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGA TGATGAGGTTTTTGAGAAGAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTG AAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAG AGTTGTTCAAACTGAAGATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCA TGATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAA GCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCC GCATCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCA GGGTCCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTG CAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGAC TTCCCCTTGTGGATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTC ACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGA CTGAGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTC GCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGCACAGATAA TAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGC CTAGAGCGGAGTGAACGGGGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGC GGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGC AGCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTG AGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATC TCCATCTCAATGACATGAAAGAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCAT CTTTGTACGGCATGGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTG CTGCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGG AATTGGCTGAGGACATGGAAATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCAT TCTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGC AAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGA CGCTGTGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGT CGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCC GAGGAGCTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGA TAGAGGCCAACCTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTG CTATTCTGCAATTATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTG CTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGA CCTTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGA GGATGCCTTCTACAGTTGGGAGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAA TCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001291157
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291157.1, NP_001278086.1
RefSeq Size	5581 bp
RefSeq ORF	4680 bp
Locus ID	1981
Protein Pathways	Viral myocarditis
Gene Summary	The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010] Transcript Variant: This variant (8) differs in the 5' UTR, includes an additional internal segment in the 5' region, which results in a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (7) is shorter at the N-terminus, and lacks an internal residue, compared to isoform 1.

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