DAP Kinase 1 (DAPK1) (NM_001288731) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAPK; ROCO3
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288731, the custom clone sequence may differ by one or more nucleotides ATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGGAACTTGGCAGTGGACAGT TTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAAAG GAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG ATCCAGCACCCCAATGTCATCACCCTGCACGAGGTCTATGAGAACAAGACGGACGTCATCCTGATCTTGG AACTCGTTGCAGGTGGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGAAGCAAC TGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACTCCCTTCAAATCGCCCACTTTGATCTT AAGCCTGAGAACATAATGCTTTTGGATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGACTTTGGGT TGGCCCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAGAGTTTGTCGCTCCTGA GATAGTCAACTATGAACCTCTTGGTCTTGAGGCAGATATGTGGAGTATCGGGGTAATAACCTATATCCTC CTAAGTGGGGCCTCCCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCCGCTGTCAACT ACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCCAAAGATTTCATAAGAAGACTTCTGGT CAAGGATCCAAAGAAGAGAATGACAATTCAAGATAGTTTGCAGCATCCCTGGATCAAGCCTAAAGATACA CAACAGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCCCGGAAAA AATGGAAACAATCCGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTCCTGTCCAGAAGTAA CATGAGTGTTGCCAGAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATCCAT GCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTCTGGGCTCATTATCCAACTATGATGTTAACC AACCCAACAAGCACGGGACACCTCCATTACTCATTGCTGCTGGCTGTGGGAATATTCAAATACTACAGTT GCTCATTAAAAGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAATGCCGTCTACTGGGCTGCT CGGCATGGCCACGTCGATACCTTGAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGT CTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTGGCTCAGTTACTGTGCAGCTT CGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACCCCCCTGCACTGTGCTGCTTGGCACGGCTAT TACTCTGTGGCCAAAGCCCTTTGTGAAGCCGGCTGTAACGTGAACATCAAGAACCGAGAAGGAGAGACGC CCCTCCTGACAGCCTCTGCCAGGGGCTACCACGACATCGTGGAGTGTCTGGCCGAACATGGAGCCGACCT TAATGCTTGCGACAAGGACGGACACATTGCCCTTCATCTGGCTGTAAGACGGTGTCAGATGGAGGTAATC AAGACTCTCCTCAGCCAAGGGTGTTTCGTCGATTATCAAGACAGGCACGGCAATACTCCCCTCCATGTGG CATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAAGCAAACTGCAATTTGGACATCTCCAA CAAGTATGGGCGAACGCCTCTGCACCTTGCGGCCAACAACGGAATCCTAGACGTGGTCCGGTATCTCTGT CTGATGGGAGCCAGCGTTGAGGCGCTGACCACGGACGGAAAGACGGCAGAAGATCTTGCTAGATCGGAAC AGCACGAGCACGTAGCAGGTCTCCTTGCAAGACTTCGAAAGGATACGCACCGAGGACTCTTCATCCAGCA GCTCCGACCCACACAGAACCTGCAGCCAAGAATTAAGCTCAAGCTGTTTGGCCACTCGGGATCCGGGAAA ACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGGAGCTTTTTCAGAAGGCGTCGGCCCAGACTGT CTTCCACCAACTCCAGCAGGTTCCCACCTTCACCCCTGGCTTCTAAGCCCACAGTCTCAGTGAGCATCAA CAACCTGTACCCAGGCTGCGAGAACGTGAGTGTGAGGAGCCGCAGCATGATGTTCGAGCCGGGTCTTACC AAAGGGATGCTGGAGGTGTTTGTGGCCCCGACCCACCACCCGCACTGCTCGGCCGATGACCAGTCCACCA AGGCCATCGACATCCAGAACGCTTATTTGAATGGAGTTGGCGATTTCAGCGTGTGGGAGTTCTCTGGAAA TCCTGTGTATTTCTGCTGTTATGACTATTTTGCTGCAAATGATCCCACGTCAATCCATGTTGTTGTCTTT AGTCTAGAAGAGCCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCCCTTG TCCCAGTTGAAGAACCCATAGCCTTCGGTGGCAAGCTGAAGAACCCACTCCAAGTTGTCCTGGTGGCCAC CCACGCTGACATCATGAATGTTCCTCGACCGGCTGGAGGCGAGTTTGGATATGACAAAGACACATCGTTG CTGAAAGAGATTAGGAACAGGTTTGGAAATGATCTTCACATTTCAAATAAGCTGTTTGTTCTGGATGCTG GGGCTTCTGGGTCAAAGGACATGAAGGTACTTCGAAATCATCTGCAAGAAATACGAAGCCAGATTGTTTC GGTCTGTCCTCCCATGACTCACCTGTGTGAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAAT GGACCCAACCAGCTGATGTCGCTGCAGCAGTTTGTGTACGACGTGCAGGACCAGCTGAACCCCCTGGCCA GCGAGGAGGACCTCAGGCGCATTGCTCAGCAGCTCCACAGCACAGGCGAGATCAACATCATGCAAAGTGA AACAGTTCAGGACGTGCTGCTCCTGGACCCCCGCTGGCTCTGCACAAACGTCCTGGGGAAGTTGCTGTCC GTGGAGACCCCACGGGCGCTGCACCACTACCGGGGCCGCTACACCGTGGAGGACATCCAGCGCCTGGTGC CCGACAGCGACGTGGAGGAGCTGCTGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAG CGGGACCATGGTGGACGTCCCAGCCCTGATCAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAG GACGAGGTGATGGTGTATGGTGGCGTGCGCATCGTGCCCGTGGAACACCTCACCCCCTTCCCATGTGGCA TCTTTCACAAGGTCCAGGTGAACCTGTGCCGGTGGATCCACCAGCAAAGCACAGAGGGCGACGCGGACAT CCGCCTGTGGGTGAATGGCTGCAAGCTGGCCAACCGTGGGGCCGAGCTGCTGGTGCTGCTGGTCAACCAC GGCCAGGGCATTGAGGTCCAGGTCCGCGGCCTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACT CGGTGTGCAGCACCATTGAGAACGTCATGGCCACCACGCTGCCAGGGCTCCTGACCGTGAAGCATTACCT GAGCCCCCAGCAGCTGCGGGAGCACCATGAGCCCGTCATGATCTACCAGCCACGGGACTTCTTCCGGGCA CAGACTCTGAAGGAAACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGCAGCATCATGT GCTTCGGGTGTCACGACGTCTACTCACAGGCCAGCCTCGGCATGGACATCCATGCATCAGACCTGAACCT CCTCACTCGGAGGAAACTGAGTCGCCTGCTGGACCCGCCCGACCCCCTGGGGAAGGACTGGTGCCTTCTC GCCATGAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAGTAACGGGGCTCCCAAGGATTTCC TCCCCAGCCCCCTCCACGCCCTGCTGCGGGAATGGACCACCTACCCTGAGAGCACAGTGGGCACCCTCAT GTCCAAACTGAGGGAGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTGTTCAAA ATCAACCTGGATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTGCAACAGCGGCACCTCTTACAATTCCA TTAGCTCTGTTGTATCCCGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001288731
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288731.1, NP_001275660.1
RefSeq Size	5760 bp
RefSeq ORF	4293 bp
Locus ID	1612
UniProt ID	P53355
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Bladder cancer, Pathways in cancer
Gene Summary	Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.