MYO6 (NM_001300899) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DFNA22; DFNB37
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300899, the custom clone sequence may differ by one or more nucleotides ATGGAGGATGGAAAGCCCGTTTGGGCGCCACACCCTACAGATGGATTTCAGATGGGCAATATTGTGGATA TTGGCCCCGACAGCTTAACAATTGAACCCTTGAATCAGAAAGGCAAGACATTTTTGGCTCTCATAAACCA AGTGTTTCCTGCAGAAGAGGACAGTAAAAAAGATGTGGAAGATAACTGTTCACTAATGTATTTAAATGAA GCCACACTGCTCCATAATATCAAAGTTCGATATAGTAAAGACAGAATTTATACATATGTCGCCAACATTC TGATTGCAGTGAATCCATACTTTGACATACCTAAAATATATTCTTCAGAAGCAATAAAGTCATATCAAGG AAAATCTCTTGGGACAAGACCACCTCATGTCTTTGCAATTGCTGATAAAGCTTTTCGAGACATGAAGGTG CTCAAGATGAGTCAGTCTATCATTGTATCTGGAGAATCAGGAGCCGGCAAAACAGAAAATACAAAATTTG TTCTAAGATACCTGACTGAATCCTATGGAACAGGTCAAGATATTGATGACAGAATTGTTGAAGCTAACCC ACTCCTAGAAGCCTTTGGAAATGCGAAGACTGTTCGCAACAATAATAGCAGTCGATTTGGGAAATTTGTA GAAATACATTTTAATGAAAAGAGCTCAGTTGTTGGAGGATTTGTTTCACATTATCTCCTAGAGAAATCTA GGATCTGTGTTCAAGGCAAAGAGGAAAGAAATTATCATATCTTTTATAGGTTGTGTGCTGGTGCTTCTGA AGATATTAGAGAAAAACTTCATTTGAGTTCACCAGATAATTTTCGGTATTTAAACCGAGGCTGCACTAGA TACTTTGCTAACAAAGAAACTGACAAACAGATTTTACAGAACCGCAAAAGTCCTGAGTACCTTAAGGCAG GTTCTATGAAAGATCCTCTGCTAGATGACCATGGTGATTTTATTAGAATGTGCACGGCTATGAAAAAAAT TGGTTTGGATGATGAAGAAAAGCTTGATCTCTTCCGGGTAGTAGCTGGCGTCCTGCACCTTGGAAATATT GATTTTGAGGAAGCTGGCAGCACTTCAGGTGGTTGTAATCTGAAGAATAAATCTGCTCAGTCTTTGGAAT ATTGTGCTGAATTACTGGGTTTGGACCAAGATGATCTTCGAGTAAGTTTGACCACAAGAGTCATGCTAAC AACAGCAGGGGGCACCAAAGGAACAGTTATAAAGGTACCTCTGAAAGTGGAGCAAGCAAACAATGCTCGT GATGCCCTGGCAAAGACAGTGTATAGCCATCTTTTTGATCATGTGGTAAACAGAGTAAATCAGTGTTTTC CTTTTGAAACATCATCCTATTTTATTGGAGTCCTAGATATTGCTGGTTTTGAGTACTTTGAGCATAACAG TTTTGAACAATTTTGCATCAACTATTGCAATGAAAAACTTCAACAATTTTTTAATGAAAGGATTCTGAAG GAGGAACAAGAACTCTATCAAAAAGAAGGTTTAGGTGTTAATGAAGTGCATTATGTGGATAATCAGGACT GTATAGATTTAATTGAAGCCAAATTAGTGGGAATACTGGATATTTTGGATGAAGAAAATCGCCTTCCCCA GCCAAGTGATCAACACTTTACATCTGCAGTTCACCAAAAGCACAAGGATCATTTTCGACTCACTATTCCC AGAAAATCTAAGCTGGCAGTTCATAGGAATATCAGAGACGACGAAGGCTTCATTATCAGGCATTTTGCGG GGGCAGTGTGCTATGAAACAACCCAGTTTGTGGAGAAAAATAATGATGCTTTACATATGTCTCTTGAATC CTTAATATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCATCCACAAATAACAACAAAGAT ACTAAACAAAAAGCAGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTTAAGACACAGTTAAATTTGC TTCTGGATAAACTTCGAAGTACTGGAGCAAGCTTTATTCGTTGCATCAAACCTAACTTAAAGATGACAAG CCACCACTTTGAAGGTGCTCAAATTCTGTCTCAGCTTCAGTGTTCAGGGATGGTGTCTGTTTTGGACTTG ATGCAGGGTGGTTACCCATCACGAGCTTCATTTCATGAACTCTACAACATGTACAAAAAGTATATGCCAG ATAAACTTGCAAGATTGGATCCAAGACTATTTTGTAAGGCTTTGTTTAAAGCTTTGGGCTTAAATGAAAA TGACTACAAGTTTGGGTTAACCAAAGTATTTTTTAGACCTGGCAAGTTTGCAGAATTTGATCAGATCATG AAGTCTGACCCTGACCACTTAGCAGAGTTGGTTAAAAGAGTCAATCACTGGCTCACATGCAGTCGCTGGA AGAAAGTTCAGTGGTGCTCACTCTCAGTCATCAAATTGAAAAACAAAATAAAATATCGAGCTGAAGCCTG CATTAAAATGCAAAAAACTATTCGAATGTGGCTTTGCAAGAGGAGACACAAACCTCGCATTGATGGTCTG GTTAAGGTGGGCACACTGAAAAAACGACTTGATAAATTTAATGAGGTAGTCAGTGTGTTGAAAGATGGAA AACCCGAGATGAATAAACAGATCAAGAATCTGGAAATTTCTATTGATACTTTGATGGCCAAAATTAAGTC CACTATGATGACGCAGGAACAAATCCAGAAAGAATATGATGCACTGGTTAAAAGCTCAGAGGAACTCCTC AGTGCATTACAGAAAAAAAAACAGCAGGAAGAGGAAGCAGAAAGGCTGAGGCGTATTCAAGAAGAAATGG AAAAGGAAAGAAAAAGACGTGAAGAAGACGAAAAACGTCGAAGAAAGGAAGAGGAGGAAAGGCGGATGAA ACTTGAGATGGAAGCAAAGAGAAAACAAGAAGAAGAAGAGAGAAAGAAAAGGGAAGATGATGAAAAACGC ATTCAAGCTGAAGTGGAGGCACAGCTGGCCCGACAGAAGGAGGAGGAATCCCAACAGCAAGCAGTTCTGG AGCAGGAGCGCAGGGACCGGGAGCTGGCCCTGAGGATTGCCCAGAGTGAAGCCGAGCTCATCAGTGATGA GGCCCAGGCCGACCTGGCGCTGCGGAGAGGTCCTGCTGTACTAGCCACCAAAGCAGCTGCTGGTACTAAG AAATATGATCTTAGTAAATGGAAATATGCAGAACTACGTGATACCATCAATACTTCTTGTGATATTGAGC TCCTGGCAGCTTGCAGAGAAGAATTTCATAGGAGACTAAAAGTGTATCATGCTTGGAAATCTAAGAACAA GAAGAGAAATACTGAAACAGAGCAACGTGCTCCAAAGTCTGTTACTGATTATGATTTTGCACCATTTTTG AACAATTCACCTCAGCAAAACCCAGCAGCTCAGATTCCTGCCAGGCAGCGGGAGATTGAAATGAACCGAC AGCAACGCTTCTTCCGCATCCCATTCATCCGCCCTGCCGACCAGTACAAAGACCCTCAGAGTAAGAAAAA AGGCTGGTGGTATGCCCATTTTGATGGACCATGGATTGCCCGGCAAATGGAACTCCATCCTGACAAGCCA CCCATCCTACTTGTGGCTGGTAAGGACGACATGGAGATGTGTGAGCTGAATCTTGAGGAGACTGGCCTGA CTCGGAAGCGTGGTGCTGAGATCTTGCCAAGACAGTTTGAAGAAATCTGGGAACGCTGTGGAGGCATCCA GTACCTTCAGAATGCGATTGAGAGCAGACAGGCTCGGCCCACCTATGCAACAGCCATGCTGCAGAGTCTG TTAAAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001300899
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300899.1, NP_001287828.1
RefSeq Size	8609 bp
RefSeq ORF	3789 bp
Locus ID	4646
UniProt ID	Q9UM54
Gene Summary	This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) lacks two in-frame exons in the 3' coding region, compared to variant 1. It encodes isoform 2 which has a shorter C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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