CNGB1 (NM_001286130) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CNCG2; CNCG3L; CNCG4; CNG4; CNGB1B; GAR1; GARP; GARP2; RCNC2; RCNCb; RCNCbeta; RP45
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286130, the custom clone sequence may differ by one or more nucleotides ATGTTGGGCTGGGTCCAGAGGGTGCTGCCTCAGCCCCCAGGGACCCCTCGGAAGACCAAGATGCAGGAGG AAGAGGAAGTGGAACCAGAGCCAGAGATGGAGGCGGAGGTGGAACCAGAACCGAATCCTGAGGAGGCCGA GACAGAGTCCGAGTCCATGCCCCCCGAAGAGTCATTCAAGGAGGAGGAAGTGGCTGTGGCAGACCCAAGC CCTCAGGAGACCAAGGAGGCTGCCCTTACTTCCACCATATCCCTCCGGGCCCAGGGCGCTGAGATTTCTG AAATGAATAGTCCCAGCCGCAGGGTACTGACCTGGCTCATGAAGGGCGTAGAGAAGGTGATCCCGCAGCC TGTTCACAGCATCACGGAGGACCCGGCTCAGATCCTGGGGCATGGCAGCACTGGGGACACAGGGTGCACA GATGAACCCAATGAGGCCCTTGAGGCCCAAGACACTAGGCCTGGGCTGCGGCTGCTTCTGTGGCTGGAGC AGAATCTGGAAAGAGTGCTTCCTCAGCCCCCCAAATCCTCTGAGGTCTGGAGAGATGAGCCTGCAGTTGC TACAGCGCCTCCAGGACGCCCCCAGGAAATGGGGCCCAAGCTGCAGGCCCGGGAGACCCCCTCCCTGCCC ACACCCATCCCCCTGCAGCCCAAGGAGGAACCCAAGGAGGCACCAGCTCCAGAGCCCCAGCCCGGCTCCC AGGCCCAGACCTCCTCCCTGCCACCAACCAGGGACCCTGCCAGGCTGGTGGCATGGGTCCTGCACAGGCT GGAGATGGCCTTGCCGCAGCCAGTGCTACATGGGAAAATAGGGGAACAGGAGCCTGACTCCCCTGGGATA TGTGATGTGCAGACCATCAGCATCCTTCCTGGAGGACAAGTGGAGCCTGACCTTGTCCTAGAGGAGGTTG AACCGCCCTGGGAGGATGCCCACCAGGATGTCAGTACCAGCCCACAGGGTACAGAGGTGGTTCCAGCTTA TGAAGAAGAGAACAAAGCTGTGGAGAAGATGCCCAGAGAGCTGTCCCGGATTGAAGAGGAGAAAGAAGAT GAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGTGACTGAGGTGCTGCTGGATAGCT GTGTGGTGTCGCAGGTGGGCGTGGGCCAGAGTGAAGAAGACGGGACCCGGCCCCAGAGCACTTCAGATCA GAAGCTGTGGGAGGAAGTTGGGGAGGAGGCCAAGAAGGAGGCTGAAGAGAAGGCCAAGGAGGAGGCCGAG GAGGTGGCTGAAGAGGAGGCTGAAAAGGAGCCCCAGGACTGGGCGGAGACCAAGGAGGAGCCTGAGGCTG AGGCCGAGGCTGCCAGTTCAGGAGTGCCTGCCACGAAACAGCACCCAGAAGTGCAGGTGGAAGATACTGA TGCTGATAGCTGCCCCCTCATGGCAGAAGAGAATCCACCCTCAACCGTGTTGCCGCCACCGTCTCCTGCC AAATCAGACACCCTTATAGTCCCAAGCTCAGCCTCGGGGACACACAGGAAGAAGCTGCCCTCTGAGGATG ATGAGGCTGAAGAGCTCAAGGCGTTGTCACCAGCAGAGTCCCCAGTGGTTGCCTGGTCTGACCCCACCAC CCCGAAGGACACTGATGGCCAGGACCGTGCGGCCTCCACGGCCAGCACAAATAGCGCCATCATCAACGAC CGGCTCCAGGAGCTGGTGAAGCTCTTCAAGGAGCGGACAGAGAAAGTGAAGGAGAAACTCATTGACCCTG ACGTCACCTCTGATGAGGAGAGCCCCAAGCCCTCCCCAGCCAAGAAAGCCCCAGAGCCAGCTCCAGACAC AAAGCCCGCTGAAGCCGAGCCAGTGGAAGAGGAGCACTATTGCGACATGCTCTGCTGCAAGTTCAAACAC CGCCCCTGGAAGAAGTACCAGTTTCCCCAGAGCATTGACCCGCTGACCAACCTGATGTATGTCCTATGGC TGTTCTTCGTGGTGATGGCCTGGAATTGGAACTGTTGGCTGATTCCCGTGCGCTGGGCCTTCCCCTACCA GACCCCGGACAACATCCACCACTGGCTGCTGATGGATTACCTATGCGACCTCATCTACTTCCTGGACATC ACCGTGTTCCAGACACGCCTGCAGTTTGTCAGAGGCGGGGACATCATTACGGACAAAAAGGACATGCGAA ATAACTACCTGAAGTCTCGCCGCTTCAAGATGGACCTGCTCAGCCTCCTGCCCTTGGATTTTCTCTATTT GAAAGTCGGTGTGAACCCCCTCCTCCGCCTGCCCCGCTGTTTAAAGTACATGGCCTTCTTCGAGTTTAAC AGCCGCCTGGAATCCATCCTCAGCAAAGCCTACGTGTACAGGGTCATCAGGACCACAGCCTACCTTCTCT ACAGCCTGCATTTGAATTCCTGTCTTTATTACTGGGCATCGGCCTATCAGGGCCTCGGCTCCACTCACTG GGTTTACGATGGCGTGGGAAACAGTTATATTCGCTGTTACTACTTTGCTGTGAAGACCCTCATCACCATC GGGGGGCTGCCTGACCCCAAGACACTCTTTGAAATTGTCTTCCAGCTGCTGAATTATTTCACGGGCGTCT TTGCTTTCTCTGTGATGATCGGACAGATGAGAGATGTGGTAGGGGCCGCCACCGCCGGACAGACCTACTA CCGCAGCTGCATGGACAGCACGGTGAAGTACATGAATTTCTACAAGATCCCCAAGTCCGTGCAGAACCGC GTCAAGACCTGGTACGAGTACACCTGGCACTCGCAAGGCATGCTGGATGAGTCAGAGCTGATGGTGCAGC TTCCAGACAAGATGCGGCTGGACCTCGCCATCGACGTGAACTACAACATCGTTAGCAAAGTCGCACTCTT TCAGGGCTGTGACCGGCAGATGATCTTTGACATGCTGAAGAGGCTTCGCTCTGTTGTCTACCTGCCCAAC GACTATGTGTGCAAGAAGGGGGAGATCGGCCGTGAGATGTACATCATCCAGGCAGGGCAAGTGCAGGTCT TGGGCGGCCCTGATGGGAAATCTGTGCTGGTGACGCTGAAAGCTGGATCTGTGTTTGGAGAAATAAGCTT GCTGGCTGTTGGGGGCGGGAACCGGCGCACGGCCAACGTGGTGGCGCACGGGTTTACCAACCTCTTCATC CTGGATAAGAAGGACCTGAATGAGATTTTGGTGCATTATCCTGAGTCTCAGAAGTTACTCCGGAAGAAAG CCAGGCGCATGCTGAGAAGCAACAATAAGCCCAAGGAGGAGAAGAGCGTGCTGATCCTTCCACCCCGGGC GGGCACCCCAAAGCTCTTCAACGCTGCCCTCGCTATGACAGGAAAGATGGGTGGCAAGGGGGCAAAAGGC GGCAAACTTGCTCACCTCCGGGCCCGGCTCAAAGAACTGGCCGCGCTGGAGGCGGCTGCAAAGCAGCAAG AGTTGGTGGAACAGGCCAAGAGCTCGCAAGACGTCAAGGGAGAGGAAGGCTCCGCCGCCCCAGACCAGCA CACGCACCCAAAGGAGGCCGCCACCGACCCACCCGCGCCCCGGACGCCCCCCGAGCCCCCGGGGTCTCCA CCGAGCTCTCCACCGCCTGCCTCCCTTGGGAGGCCGGAGGGAGAGGAGGAGGGGCCGGCCGAGCCCGAAG AGCACTCGGTGAGGATCTGCATGAGCCCGGGCCCGGAGCCGGGAGAGCAGATCCTGTCGGTGAAGATGCC GGAGGAAAGGGAGGAGAAGGCGGAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286130
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286130.1, NP_001273059.1
RefSeq Size	5645 bp
RefSeq ORF	3738 bp
Locus ID	1258
UniProt ID	Q14028
Protein Families	Druggable Genome, Ion Channels: Cyclic nucleotide gated
Protein Pathways	Olfactory transduction
Gene Summary	In humans, the rod photoreceptor cGMP-gated cation channel helps regulate ion flow into the rod photoreceptor outer segment in response to light-induced alteration of the levels of intracellular cGMP. This channel consists of two subunits, alpha and beta, with the protein encoded by this gene representing the beta subunit. Defects in this gene are a cause of cause of retinitis pigmentosa type 45. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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