AMBRA1 (NM_001300731) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DCAF3; WDR94
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300731, the custom clone sequence may differ by one or more nucleotides ATGAAGGTTGTCCCAGAAAAGAATGCTGTCCGGATACTCTGGGGGCGAGAACGGGGTGCTCGGGCCATGG GAGCTCAGCGGCTTCTGCAGGAGCTGGTAGAAGATAAAACCCGGTGGATGAAATGGGAGGGCAAGAGAGT AGAACTGCCGGATAGTCCACGCTCTACCTTCTTATTGGCCTTCAGCCCAGACAGGACTCTCTTAGCCTCC ACCCATGTGAACCATAATATCTATATTACGGAGGTGAAGACTGGCAAGTGTGTTCATTCCCTGATTGGAC ACCGCCGTACTCCATGGTGTGTCACTTTTCATCCCACCATCTCAGGCCTTATTGCTTCTGGCTGCCTAGA TGGGGAGGTTAGGATTTGGGATTTACACGGTGGCAGTGAAAGCTGGTTCACAGATAGCAACAATGCCATT GCCTCCCTGGCTTTCCACCCTACGGCTCAGCTCCTGCTGATTGCCACTGCCAATGAGATCCACTTCTGGG ACTGGAGTCGACGGGAACCCTTTGCTGTGGTGAAGACAGCTAGTGAGATGGAACGGGTCCGTCTGGTGAG ATTTGATCCACTTGGACACTACTTACTCACAGCAATTGTTAACCCCTCTAATCAACAGGGTGATGACGAA CCAGAGATCCCCATAGATGGAACAGAATTATCCCACTACCGTCAGCGTGCCCTCCTGCAATCACAGCCAG TTCGCCGGACGCCTCTCCTCCACAATTTCCTGCACATGCTGTCCTCCCGCTCTTCTGGCATCCAGGTGGG AGAGCAAAGCACAGTGCAAGATTCTGCTACCCCCTCACCCCCACCGCCTCCCCCTCAGCCCTCCACGGAG CGCCCCAGGACTTCCGCTTACATCAGGCTCCGACAGCGGGTCAGTTACCCCACAGCTGAGTGCTGCCAGC ACCTTGGGATCCTGTGCCTTTGCAGCCGCTGCTCTGGCACTCGAGTTCCTTCCCTCTTGCCACACCAGGA CAGTGTCCCCCCTGCTTCTGCCAGAGCTACTACCCCTTCCTTTTCTTTTGTACAGACCGAGCCCTTCCAT CCCCCGGAGCAGGCCTCGTCAACGCAGCAGGACCAGGGCCTCCTGAACCGGCCGTCTGCCTTCAGTACAG TCCAGAGCAGCACTGCCGGCAACACGCTCCGCAACCTCAGTCTGGGTCCTACCCGCCGCTCTTTGGGAGG GCCTCTGTCTAGCCACCCTTCTAGGTATCACCGAGAAATAGCTCCTGGGTTGACAGGATCTGAGTGGACC CGGACAGTACTCAGTCTGAACTCCCGCTCTGAGGCGGAATCCATGCCCCCGCCCAGAACCAGTGCCTCTT CGGTGAGTTTGCTGTCTGTGCTGAGACAGCAGGAAGGTGGCTCTCAGGCATCTGTGTACACTTCAGCCAC AGAAGGGAGGGGTTTTCCGGCATCAGGGTTGGCAACTGAGTCAGATGGAGGGAATGGCTCCAGCCAAAAC AACTCGGGCAGCATTCGCCATGAGCTTCAGTGTGACCTGAGACGCTTCTTTCTGGAGTATGACCGGCTTC AGGAGCTGGATCAGAGCCTGAGTGGGGAAGCTCCCCAGACCCAACAGGCCCAGGAAATGCTCAACAATAA CATTGAATCTGAGAGGCCAGGCCCTTCCCACCAGCCCACCCCACACAGCAGTGAGAACAACTCCAACCTG TCCCGTGGCCACCTGAATCGCTGTCGTGCTTGCCACAATCTCCTGACCTTCAACAACGATACCCTGCGCT GGGAAAGAACCACACCTAACTACTCCTCTGGCGAGGCTAGTTCCTCTTGGCAGGTCCCCAGCTCCTTTGA GAGTGTGCCATCAAGTGGCAGCCAGTTGCCACCTCTCGAGCGGACTGAGGGCCAAACGCCCAGCTCCAGC AGGCTGGAGTTGAGCAGCTCTGCTAGTCCGCAGGAGGAGAGGACTGTGGGGGTGGCCTTTAACCAGGAGA CAGGCCACTGGGAAAGAATTTACACCCAGTCCAGCAGATCTGGAACTGTGTCACAGGAGGCCTTACATCA GGATATGCCTGAGGAGAGCTCTGAGGAGGATTCACTCAGGAGGAGGCTGCTGGAATCTTCCCTCATTTCA TTATCCCGTTATGATGGAGCAGGATCCAGAGAGCACCCAATTTACCCAGACCCAGCGAGGGACAATGGTG ACAGATCCAGGCACCGAGCTCCACGCAATGCCCGGATGTCTGCACCTTCGCTTGGACGCTTTGTCCCAAG GCGTTTCTTGCTGCCTGAGTACTTGCCTTATGCTGGGATTTTTCATGAACGTGGACAGCCTGGCTTGGCT ACTCACTCTTCTGTTAACAGGGTCCTGGCAGGGGCAGTGATCGGTGATGGACAGTCTGCTGTGGCCAGTA ACATTGCCAATACTACCTACCGGCTCCAGTGGTGGGACTTCACTAAGTTTGACCTCCCTGAAATCAGTAA TGCTTCCGTGAATGTGCTGGTGCAGAACTGCAAGATCTACAATGATGCCAGCTGTGACATTTCTGCAGAT GGCCAGCTCCTGGCAGCTTTCATCCCCAGCAGCCAGAGGGGCTTTCCTGATGAAGGCATCCTGGCAGTGT ACTCCCTGGCCCCCCATAACCTGGGCGAAATGCTCTACACCAAGCGATTTGGTCCCAATGCCATTTCGGT GAGCCTGTCCCCAATGGGCAGATATGTAATGGTGGGCTTGGCCTCACGAAGGATCCTGCTGCACCCCTCC ACAGAGCACATGGTGGCCCAGGTCTTCAGGCTGCAACAGGCCCATGGTGGAGAGACCTCCATGAGGAGAG TTTTCAACGTCCTTTATCCCATGCCTGCCGACCAGCGGAGACATGTCAGTATCAACTCTGCCCGTTGGCT GCCTGAGCCAGGGCTTGGCTTGGCCTATGGTACTAACAAAGGAGACCTGGTGATCTGCCGACCAGAGGCC TTAAACTCTGGTGTTGAGTACTACTGGGACCAGCTGAACGAGACGGTCTTCACTGTCCATTCCAACAGCA GGAGCAGCGAGCGGCCTGGAACCAGCAGAGCCACATGGAGGACAGACAGAGACATGGGGCTGATGAATGC CATTGGGCTTCAGCCCCGGAACCCTGCCACCTCAGTGACATCTCAGGGCACCCAGACTCTGGCCCTTCAG CTGCAGAATGCCGAAACACAGACTGAGAGGGAGGTGCCGGAGCCAGGGACAGCCGCCTCAGGTCCTGGTG AAGGTGAGGGTTCAGAGTATGGTGCCAGTGGAGAAGATGCGCTCAGCAGGATCCAGAGGCTGATGGCGGA GGGCGGCATGACAGCCGTGGTGCAGCGGGAGCAGAGCACCACCATGGCCTCCATGGGCGGCTTCGGCAAC AACATCATCGTCAGCCACCGCATTCACCGCAGCTCTCAGACGGGCACTGAACCTGGTGCCGCCCACACCT CCTCACCCCAGCCCTCCACCTCTCGGGGACTGCTCCCAGAGGCCGGGCAACTGGCAGAGCGAGGCCTAAG CCCCCGGACAGCTTCCTGGGACCAGCCTGGTACCCCTGGGCGGGAGCCAACCCAGCCAACCCTGCCCTCT TCCTCCCCTGTCCCCATTCCTGTTTCCCTTCCCAGCGCTGAGGGACCAACCCTCCACTGCGAGTTGACCA ATAACAACCACCTTCTGGATGGTGGCAGCAGCAGGGGGGACGCTGCAGGCCCTAGGGGAGAACCACGGAA CAGGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001300731
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300731.1, NP_001287660.1
RefSeq Size	5126 bp
RefSeq ORF	3717 bp
Locus ID	55626
UniProt ID	Q9C0C7
Gene Summary	Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) retains an alternate in-frame intron and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) contains an alternate internal segment and lacks two other alternate internal segments compared to isoform 1.

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