TReP132 (TRERF1) (NM_001297573) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCAR2; dJ139D8.5; HSA277276; RAPA; TReP-132; TREP132
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001297573, the custom clone sequence may differ by one or more nucleotides ATGGGTGACCAGCAACTGTACAAGACCAACCATGTGGCCCATGGTAGTGAGAACCTTTTCTACCAACAGC CACCACTTGGCGTCCACAGCGGGCTGAACCACAACTATGGGAATGCAGTTACAGGGGGCGGAATGGATGC CCCTCAGGCCTCGCCAATCTCCCCCCACTTCCCTCAAGATACACGGGATGGTCTGGGCTTGCCTGTTGGC TCCAAAAACCTTGGCCAAATGGATACCTCGAGGCAGGGAGGGTGGGGAAGTCATGCAGGGCCTGGAAACC ATGTCCAGCTACGTGGAAACCTGGCCAACTCAAACATGATGTGGGGGGCACCAGCCCAGGCTGAGCCCAC TGATGGCTACCAATACACCTACTCCCAGGCCAGCGAGATCCGGACCCAGAAGCTTACCAGCGGTGTCTTA CACAAGCTGGACTCTTTCACCCAGGTGTTTGCCAACCAAAACCTGCGAATTCAGGTCAACAATATGGCCC AGGTGCTGCACACTCAGTCAGCAGTGATGGATGGAGCCCCTGACAGTGCTCTCCGCCAGCTGCTGTCTCA GAAGCCCATGGAGCCCCCAGCACCGGCTATCCCTTCCCGCTACCAGCAGGTGCCCCAGCAGCCTCACCCT GGTTTCACTGGTGGGCTGTCCAAACCAGCTCTTCAGGTCGGGCAGCACCCTACCCAAGGGCACCTGTATT ATGACTACCAGCAGCCTCTGGCTCAGGTGCCAGTGCAGGGAGGACAGCCACTGCAGGCCCCACAGATGCT GTCACAGCACATGCAACAGATGCAGCAGCACCAGTATTACCCACCGCAGCAACAGCAGCAAGCCGGGCAA CAGCGTATCTCCATGCAAGAAATACAGACGCAGCCGCAACAAATTCGCCCATCACAGCCACAGCCGCCGC CACAGCAGCAGCAGCCGCAGCAGCTACAGCTGCAGCAGCGGCAGGGTTCAATGCAGATACCTCAGTATTA TCAGCCCCAACCCATGATGCAGCACTTGCAAGAGCAGCAGCAGCAACAGATGCACCTGCAGCCTCCTTCT TATCACAGGGACCCTCACCAGTATACCCCAGAGCAGGCACACACTGTCCAGCTGATTCCCCTGGGCTCCA TGTCCCAGTACTACTACCAGGAGCCCCAGCAGCCCTACAGCCACCCCCTCTACCAGCAGAGCCACCTGTC CCAGCACCAGCAGCGTGAGGACAGTCAGCTGAAGACCTACTCTAGTGACAGACAGGCCCAGGCCATGCTG AGCTCCCATGGGGACCTGGGGCCTCCTGACACAGGAATGGGAGACCCAGCGAGCTCAGATCTGACCCGGG TCAGCAGCACCCTCCCCCATCGCCCCCTCCTATCCCCCAGTGGGATCCACCTCAACAACATGGGGCCTCA GCATCAGCAGCTGTCTCCCAGTGCCATGTGGCCCCAGATGCACCTACCTGATGGGAGAGCCCAGCCAGGG TCCCCTGAGTCAAGTGGCCAACCCAAAGGAGCGTTTGGGGAGCAGTTTGATGCCAAGAACAAGCTGACAT GCTCCATCTGCCTGAAGGAGTTCAAGAACCTGCCTGCCCTGAATGGCCACATGCGGTCCCACGGGGGAAT GAGGGCCTCCCCCAACCTCAAACAGGAGGAAGGAGAGAAGGTCCTGCCGCCTCAGCCCCAGCCACCACTG CCGCCTCCGCCTCCGCCTCCGCCGCCACCACAGCTCCCTCCCGAGGCAGAAAGCCTCACGCCTATGGTCA TGCCCGTGTCTGTCCCTGTCAAGCTTCTCCCGCCCAAGCCCAGCTCTCAGGGGTTCACCAACAGCACCGT TGCCGCCCCCTCCGCCAGAGACAAGCCAGCCAGCTCGATGTCGGACGACGAGATGCCTGTGCTCGTGAGG ATGACCCTCTCTCCCCCACACTCACCCCAAGGGGCTGCCCCCCGCACGCCTGCTGAAATCCCCAGGAAGC ATCAGCCGAGTGTGCCCAAAGCCGAGGAGCCCCTCAAGACCGTGCAGGAGAAGAAAAAGTTCCGGCACCG GCCGGAACCTCTCTTCATCCCGCCGCCGCCCTCCTACAACCCGAACCCCGCTGCCTCCTACTCGGGCGCC ACCCTGTACCAGAGCCAGCTGCGCTCCCCGCGCGTCCTCGGGGACCACCTGCTCCTGGACCCCACCCACG AGCTGCCCCCTTACACGCCCCCACCCATGCTGAGCCCGGTGCGCCAGGGCTCGGGGCTCTTCAGCAATGT CCTCATCTCCGGCCACGGCCCTGGCGCCCACCCGCAGCTGCCCCTGACGCCCCTGACGCCCACACCACGG GTGCTGCTGTGTCGCTCCAACAGCATCGATGGCAGCAACGTGACGGTCACCCCAGGGCCTGGAGAGCAGA CTGTAGATGTTGAACCACGCATCAACATTGGCTTGAGATTCCAAGCAGAAATCCCTGAACTCCAAGATAT CTCTGCCCTGGCCCAGGACACACACAAGGCCACACTGGTATGGAAGCCCTGGCCAGAACTAGAAAACCAT GACCTCCAGCAAAGAGTGGAGAATCTTCTGAATTTGTGCTGTTCCAGTGCATTGCCAGGTGGAGGGACCA ATTCTGAATTTGCTTTGCACTCTCTGTTTGAGGCCAAAGGTGATGTGATGGTTGCTCTGGAAATGCTGCT ACTGCGGAAGCCTGTCAGGTTAAAATGTCATCCTTTAGCAAATTACCACTATGCCGGTTCGGACAAGTGG ACCTCCCTAGAAAGAAAACTGTTTAACAAAGCACTAGCCACTTACAGCAAAGACTTTATTTTTGTACAGA AGATGGTGAAGTCCAAGACGGTGGCTCAGTGCGTGGAGTACTACTACACGTGGAAAAAGATCATGCGGCT GGGGCGGAAACACCGGACACGCCTGGCAGAAATCATCGACGATTGTGTGACAAGTGAAGAAGAAGAAGAG TTAGAGGAGGAGGAGGAGGAGGACCCGGAAGAAGATAGGAAATCCACAAAAGAAGAAGAGAGTGAGGTGC CGAAGTCCCCGGAGCCACCACCCGTCCCCGTCCTGGCTCCCACGGAGGGGCCGCCCCTGCAGGCCCTGGG CCAGCCCTCAGGCTCCTTCATCTGTGAAATGCCCAACTGTGGGGCTGTGTTCAGCTCCCGACAGGCACTG AATGGCCATGCCCGCATCCACGGGGGCACCAACCAGGTGACCAAGGCCCGAGGTGCCATCCCCTCTGGGA AGCAGAAGCCTGGTGGCACCCAGAGTGGGTACTGTTCGGTAAAGAGCTCACCCTCTCACAGCACCACCAG CGGCGAGACAGACCCCACCACCATCTTCCCCTGCAAGGAGTGTGGCAAAGTCTTCTTCAAGATCAAAAGC CGAAATGCACACATGAAAACTCACAGGCAGCAGGAGGAACAACAGAGGCAAAAGGCTCAGAAGGCGGCTT TTGCAGCTGAGATGGCAGCCACGATTGAGAGGACTACGGGGCCCGTGGGGGCGCCGGGGCTGCTGCCCCT GGACCAGCTGAGTCTGATCAAACCCATCAAGGATGTGGACATCCTCGACGACGACGTCGTCCAGCAGTTG GGAGGTGTCATGGAAGAGGCTGAAGTTGTGGACACCGATCTTCTCTTGGATGATCAAGATTCAGTCTTGC TTCAGGGTGACGCAGAACTATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001297573
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297573.1, NP_001284502.1
RefSeq Size	7644 bp
RefSeq ORF	3663 bp
Locus ID	55809
UniProt ID	Q96PN7
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	This gene encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1) represents the longer transript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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