Phospholipase C beta 2 (PLCB2) (NM_001284297) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PLC-beta-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284297, the custom clone sequence may differ by one or more nucleotides ATGTCTCTGCTCAACCCTGTCCTGCTGCCCCCCAAGGTGAAGGCCTATCTGAGCCAAGGGGAGCGCTTCA TCAAATGGGATGATGAAACTACAGTTGCCTCTCCAGTTATCCTCCGTGTGGATCCTAAGGGCTACTACTT ATACTGGACGTATCAAAGTAAGGAGATGGAGTTTCTGGATATCACCAGCATCCGGGATACTCGCTTTGGG AAGTTTGCCAAGATGCCCAAGAGCCAGAAGCTCCGGGACGTCTTCAACATGGACTTTCCTGATAACAGTT TCCTGCTGAAGACACTCACGGTGGTGTCCGGCCCGGACATGGTGGACCTCACCTTCCACAACTTCGTCTC CTACAAGGAGAACGTGGGCAAGGCCTGGGCTGAGGACGTACTGGCCCTAGTCAAACATCCGCTGACGGCC AACGCCTCCCGCAGCACCTTCCTGGACAAGATCCTTGTGAAGCTCAAGATGCAGCTCAACTCTGAAGGGA AGATTCCGGTGAAGAACTTTTTCCAGATGTTTCCTGCTGACCGCAAGCGGGTGGAAGCTGCTCTCAGTGC CTGCCACCTCCCCAAAGGCAAAAATGACGCCATCAATCCTGAGGACTTCCCAGAACCTGTCTACAAGAGT TTCCTCATGAGCCTCTGTCCTCGGCCAGAAATAGATGAGATCTTCACTTCTTACCATGCTAAGGCCAAAC CCTACATGACGAAGGAGCACCTGACCAAATTCATCAACCAGAAACAGCGGGACTCCCGGCTTAACTCCCT GCTGTTCCCGCCAGCACGGCCTGACCAGGTGCAGGGCCTCATCGACAAGTATGAGCCCAGTGGCATCAAT GCACAGAGGGGCCAGCTGTCACCTGAAGGCATGGTCTGGTTTCTCTGTGGGCCAGAGAACAGCGTGCTGG CCCAGGACAAGCTGCTGCTCCACCACGACATGACGCAGCCACTCAATCATTACTTCATCAACTCGTCCCA CAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTCTCCTCGGCTGAGATGTACCGCCAGGTGCTGCTC TCTGGCTGCCGTTGCGTGGAGCTAGACTGCTGGAAGGGGAAACCCCCTGACGAGGAGCCCATTATCACCC ATGGCTTCACCATGACCACAGACATCTTCTTCAAAGAAGCAATTGAGGCTATTGCAGAAAGCGCCTTTAA GACCTCCCCCTATCCCATCATCCTGTCGTTTGAGAACCATGTGGACTCACCCCGCCAGCAGGCTAAGATG GCTGAGTATTGCCGGACGATCTTTGGGGATATGCTGCTCACAGAGCCCCTGGAAAAGTTCCCACTAAAAC CAGGTGTCCCCCTGCCCAGCCCTGAGGATCTCAGGGGCAAGATCCTCATCAAGAACAAGAAGAACCAGTT TTCTGGCCCCACCTCCTCCAGTAAGGATACTGGTGGGGAGGCTGAGGGCAGCAGCCCACCCAGTGCCCCT GCAGTGTGGGCTGGCGAGGAAGGGACTGAGCTGGAGGAGGAGGAGGTGGAAGAGGAAGAGGAGGAGGAGT CAGGAAACCTGGATGAAGAAGAGATTAAGAAGATGCAGTCGGATGAGGGCACAGCGGGCCTGGAAGTGAC GGCTTATGAGGAGATGTCCAGCCTAGTCAATTACATCCAGCCCACCAAGTTCGTCTCCTTTGAGTTCTCT GCCCAAAAGAACCGAAGTTATGTCATCTCGTCCTTCACAGAGCTCAAGGCATATGACCTGCTCTCCAAGG CCTCGGTGCAGTTTGTGGACTACAACAAGCGCCAGATGAGCCGCATTTACCCCAAGGGAACCCGCATGGA CTCCTCCAACTACATGCCCCAGATGTTCTGGAATGCTGGATGCCAGATGGTTGCCCTCAACTTCCAGACG ATGGACTTGCCCATGCAGCAGAACATGGCAGTATTTGAGTTCAACGGGCAGAGCGGCTACCTCCTCAAGC ATGAGTTCATGCGCCGGCCGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCATCGACGTGGTGGTGGC CACCACCCTTTCCATTACGGTGATCTCTGGGCAGTTCCTGTCAGAACGCAGCGTGCGCACCTATGTAGAA GTGGAGCTGTTTGGCCTTCCTGGGGACCCCAAGAGGCGCTATCGAACTAAGCTGTCACCCAGTACTAACT CCATCAATCCTGTCTGGAAGGAGGAGCCCTTTGTCTTTGAGAAGATCTTGATGCCTGAGCTGGCCTCCCT CAGAGTGGCTGTGATGGAGGAAGGCAACAAGTTTCTTGGACACCGCATCATCCCCATCAATGCCCTAAAT TCTGGGTACCACCACCTGTGCCTGCACAGTGAGAGCAACATGCCCCTCACCATGCCTGCGCTCTTCATCT TCCTGGAGATGAAGGACTACATACCTGGTGCTTGGGCAGATCTCACTGTGGCCCTCGCCAACCCCATTAA GTTCTTCAGTGCCCATGACACGAAGTCTGTGAAGCTCAAGGAGGCCATGGGAGGTCTGCCTGAGAAGCCC TTCCCACTGGCGAGTCCAGTTGCCAGCCAGGTCAATGGGGCGTTGGCCCCAACGAGCAATGGGTCACCAG CAGCCAGGGCCGGGGCCAGGGAAGAGGCTATGAAAGAAGCTGCGGAGCCGCGGACCGCCAGCCTGGAGGA GCTCCGGGAGCTAAAGGGCGTGGTGAAGCTGCAGCGGCGGCACGAGAAGGAGCTGCGAGAGTTGGAGCGG CGCGGAGCGCGGCGCTGGGAGGAGCTGCTGCAGCGGGGCGCGGCGCAGCTGGCGGAGCTCGGGCCACCGG GCGTGGGGGGCGTCGGGGCCTGCAAGCTCGGTCCCGGCAAGGGCTCTCGCAAGAAGAGGAGCCTGCCCCG CGAGGAGAGCGCCGGAGCCGCGCCGGGCGAGGGCCCTGAGGGCGTGGACGGGCGCGTGCGGGAGCTGAAA GACAGGCTGGAGCTGGAGCTGCTGCGGCAGGGCGAGGAGCAGTACGAGTGCGTTCTGAAGCGCAAGGAGC AGCACGTGGCCGAGCAAATCTCCAAAATGATGGAGCTGGCCAGAGAGAAACAGGCGGCAGAGCTGAAGGC CCTGAAGGAGACGTCGGAGAACGACACCAAAGAGATGAAGAAAAAGCTGGAGACAAAGAGACTGGAGCGG ATCCAGGGCATGACCAAAGTCACCACAGACAAGATGGCCCAGGAGAGGTTGAAGAGAGAGATTAACAACT CCCACATCCAGGAAGTAGTGCAGGTGATCAAGCAGATGACGGAGAACTTGGAGAGGCACCAGGAGAAGCT GGAGGAGAAGCAGGCGGCTTGCCTGGAACAGATACGGGAGATGGAAAAGCAGTTCCAGAAGGAGGCGCTG GCAGAGTACGAGGCCAGGATGAAGGGTCTGGAGGCAGAGGTGAAGGAGTCGGTGAGGGCCTGCCTCAGGA CCTGCTTTCCCTCCGAGGCCAAGGACAAGCCTGAGAGGGCCTGCGAGTGCCCCCCAGAGCTGTGTGAGCA GGACCCACTCATAGCAAAGGCAGATGCCCAGGAGAGCCGCCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001284297
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284297.1, NP_001271226.1
RefSeq Size	4682 bp
RefSeq ORF	3546 bp
Locus ID	5330
UniProt ID	Q00722
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Taste transduction, Vascular smooth muscle contraction, Wnt signaling pathway
Gene Summary	The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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